Effect of Serum on Electrochemical Detection of Bioassays Having Ag Nanoparticle Labels.
Effect of Serum on Electrochemical Detection of Bioassays Having Ag Nanoparticle Labels.
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DOI：
10.1021/acssensors.1c00446
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发表时间：
2021-05-28
期刊：
影响因子：
8.9
通讯作者：
Crooks RM
中科院分区：
文献类型：
--
作者：
Pollok NE;Peng Y;Rabin C;Richards I;Crooks RM
研究了血清对纳米银(AgNP)检测标记生物检测的影响。对心力衰竭标志物NT-proBNP的模型试验和抗原特异性夹心生物试验进行了检测。在这两种情况下，AgNP标记都连接到检测抗体上。电化学检测采用电流交换/阳极溶出伏安法，其中AU3+与AgNP标记交换。使用纸基电极平台进行分析。在检测之前和检测期间，生物检测暴露在不同的血清条件下。本文报告了三个重要的结果。首先，模型和抗原特异性分析都可以在未稀释的血清中形成，没有血清成分的可检测到的干扰。其次，为了获得最大可能的电化学信号，在不进行洗涤的情况下，在电化学检测过程中可以保留在分析缓冲液中的血清的最高百分比为0.25%。当血清中存在0.50%或更多的血清时，检测结果不准确。第三，抑制血清中电交换的因素可能与生物分子在AgNP标记上的表面吸附、血清成分对Au~(3+)的络合或两者兼而有之。本文的结果为在生物流体中使用金属NP标记进行电化学检测提供了一般性的指导。
The effect of serum on electrochemical detection of bioassays having silver nanoparticle (AgNP) detection labels was investigated. Both a model assay and an antigen-specific sandwich bioassay for the heart-failure marker NT-proBNP were examined. In both cases, the AgNP labels were conjugated to a detection antibody. Electrochemical detection was carried out using a galvanic exchange/anodic stripping voltammetry method in which Au3+ exchanges with AgNP labels. The assays were carried out using a paper-based electrode platform. The bioassays were exposed to different serum conditions prior to and during detection. There are three important outcomes reported in this article. First, both the model and antigen-specific assays could be formed in undiluted serum with no detectable interferences from the serum components. Second, to achieve the maximum possible electrochemical signal, the highest percentage of serum that can remain in an assay buffer during electrochemical detection is 0.25% when no washing is performed. The assay results are rendered inaccurate when 0.50% or more of serum is present. Third, the factors inhibiting galvanic exchange in serum probably relate to surface adsorption of biomolecules onto the AgNP labels, chelation of Au3+ by serum components, or both. The results reported here provide general guidance for using metal NP labels for electrochemical assays in biofluids.
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影响因子：
8.4
作者：
Chekin, Fereshteh;Vasilescu, Alina;Szunerits, Sabine
通讯作者：
Szunerits, Sabine
影响因子：
8.9
作者：
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影响因子：
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作者：
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DOI：
10.1109/jtehm.2017.2740920
发表时间：
2017
影响因子：
3.4
作者：
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通讯作者：
Crooks RM
影响因子：
4.6
作者：
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通讯作者：
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