YKL-40 is highly expressed in the epicardial adipose tissue of patients with atrial fibrillation and associated with atrial fibrosis.
YKL-40 is highly expressed in the epicardial adipose tissue of patients with atrial fibrillation and associated with atrial fibrosis.
复制标题
YKL-40 在心房颤动患者的心外膜脂肪组织中高表达并与心房纤维化相关
DOI：
10.1186/s12967-018-1598-0
复制
发表时间：
2018-08-15
影响因子：
7.4
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Wang Q;Shen H;Min J;Gao Y;Liu K;Xi W;Yang J;Yin L;Xu J;Xiao J;Wang Z
研究背景YKL-40（CHI 3L 1）是一种新的炎症、组织重塑、纤维化和心血管疾病的生物标志物。我们研究了心房颤动（AF）患者心外膜脂肪组织（EAT）中YKL-40表达与心房纤维化之间的关系。方法收集接受冠状动脉旁路移植术患者的血液样本、皮下脂肪组织（SAT）、心旁脂肪组织（PAT）、EAT和相邻心房心肌。结果窦性心律（SR）组与房颤组血清YKL-40水平无显著性差异（P= 0.145）。实时荧光定量PCR结果显示，EAT中YKL-40（CHI 3L 1）mRNA水平显著高于AF患者SAT、PAT和SR患者EAT（P < 0.001）。我们通过免疫组化发现YKL-40蛋白水平的类似结果。Masson染色显示AF组纤维化程度明显高于SR组（P< 0.001）。Western blotting结果显示，房颤组Ⅰ型胶原表达明显高于对照组（P= 0.039）。YKL-40 mRNA表达与心房肌胶原体积分数呈直线相关（y = 3.576x +26.205，P < 0.001）。多因素线性回归分析显示，体质量指数（BMI）是影响EAT中YKL-40表达的独立危险因素（β = 0.328，P = 0.011）。
BackgroundYKL-40 (CHI3L1) is a novel biomarker for inflammation, tissue remodeling, and fibrosis, as well as cardiovascular diseases. We investigated the association between YKL-40 expression in epicardial adipose tissue (EAT) and atrial fibrosis in patients with atrial fibrillation (AF).MethodsBlood samples, subcutaneous adipose tissue (SAT), paracardial adipose tissue (PAT), EAT, and adjacent atrial myocardium were acquired from patients receiving coronary artery bypass grafts. The patients were divided into the AF group (n = 28) and the sinus rhythm (SR) group (n = 36).ResultsWe did not detect a significant difference in the serum YKL-40 levels in the SR and AF groups (P= 0.145). Quantitative real-time PCR showed that YKL-40 (CHI3L1) mRNA levels in the EAT were significantly higher than in the SAT or PAT of AF patients, or the EAT of SR patients (AllP< 0.001). We found similar results for YKL-40 protein levels by immunohistochemistry. Masson staining showed significantly more fibrosis in AF patients than in SR patients (P< 0.001). Western blotting indicated that AF patients had significantly higher expression of collagen I (P= 0.039). We found a linear relationship between YKL-40 mRNA expression and the collagen volume fraction of the atrial myocardium (y = 3.576x + 26.205,P< 0.001). Multivariate linear regression analysis revealed that body mass index is an independent risk factor for YKL-40 expression in EAT (β = 0.328,P= 0.011).ConclusionsYKL-40, which is highly expressed in the EAT of patients with AF, is affected by body mass index and associated with atrial fibrosis, which may contribute to the development of AF.
登录
查看更多内容
影响因子：
9.3
作者：
Jalife J;Kaur K
通讯作者：
Kaur K
影响因子：
39.3
作者：
Kastrup, Jens;Johansen, Julia S.;Gluud, Christian
通讯作者：
Gluud, Christian
DOI：
10.1016/j.bbrc.2009.08.024
发表时间：
2009-10-23
影响因子：
3.1
作者：
Iwata, Takeo;Kuwajima, Masamichi;Yoshimoto, Katsuhiko
通讯作者：
Yoshimoto, Katsuhiko
影响因子：
37.8
作者：
Chugh SS;Havmoeller R;Narayanan K;Singh D;Rienstra M;Benjamin EJ;Gillum RF;Kim YH;McAnulty JH Jr;Zheng ZJ;Forouzanfar MH;Naghavi M;Mensah GA;Ezzati M;Murray CJ
通讯作者：
Murray CJ
影响因子：
24
作者：
Al Chekakie, M. Obadah;Welles, Christine C.;Akar, Joseph G.
通讯作者：
Akar, Joseph G.