Tracking T and B cells from two-photon microscopy imaging using constrained SMC clusters

Tracking T and B cells from two-photon microscopy imaging using constrained SMC clusters
复制标题

使用受限 SMC 簇通过双光子显微镜成像追踪 T 细胞和 B 细胞

DOI:
--
复制
发表时间:
2011
影响因子:
1.9
通讯作者:
C. Tadokoro
C. Tadokoro
中科院分区:
--
文献类型:
--
作者:
D. Olivieri;J. Faro;Iván Gómez;C. Tadokoro

文献摘要

参考文献

被引文献

相似文献

摘要本文介绍了一种新的软件算法,称为约束顺序蒙特卡罗(SMC)集群,用于跟踪一个大的收集单个细胞从活体双光子显微镜图像序列。我们展示了如何我们的方法和软件工具,在Python中实现,是有用的量化的T和B淋巴细胞参与免疫反应与淋巴细胞在非免疫条件下的运动。我们描述了我们的算法背后的理论,并简要讨论了我们的软件的架构。最后,我们展示了软件的功能和实用性,通过将其应用于两个实际的例子,从视频显示淋巴细胞的运动在淋巴结的B细胞区(滤泡)和T细胞区。
Summary This paper describes a novel software algorithm, called constrained Sequential Monte Carlo (SMC) clusters, for tracking a large collection of individual cells from intra-vital two-photon microscopy image sequences. We show how our method and software tool, implemented in python, is useful for quantifying the motility of T and B lymphocytes involved in an immune response vs lymphocytes under non immune conditions. We describe the theory behind our algorithm and briefly discuss the architecture of our software. Finally, we demonstrate both the functionality and utility of software by applying it to two practical examples from videos displaying lymphocyte motility in B cell zones (follicles) and T cell zones of lymph nodes.
DOI: 10.1126/science.2321027
发表时间: 1990-04-06
期刊: SCIENCE
影响因子: 56.9
作者:
DENK, W;STRICKLER, JH;WEBB, WW
通讯作者: WEBB, WW
DOI: 10.1016/j.immuni.2007.04.008
发表时间: 2007-05-01
期刊: IMMUNITY
影响因子: 32.4
作者:
Hauser, Anja E.;Junt, Tobias;Haberman, Ann M.
通讯作者: Haberman, Ann M.