Tracking T and B cells from two-photon microscopy imaging using constrained SMC clusters
Tracking T and B cells from two-photon microscopy imaging using constrained SMC clusters
复制标题
使用受限 SMC 簇通过双光子显微镜成像追踪 T 细胞和 B 细胞
DOI:
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发表时间:
2011
影响因子:
1.9
通讯作者:
C. Tadokoro
中科院分区:
文献类型:
--
作者:
D. Olivieri;J. Faro;Iván Gómez;C. Tadokoro
Summary This paper describes a novel software algorithm, called constrained Sequential Monte Carlo (SMC) clusters, for tracking a large collection of individual cells from intra-vital two-photon microscopy image sequences. We show how our method and software tool, implemented in python, is useful for quantifying the motility of T and B lymphocytes involved in an immune response vs lymphocytes under non immune conditions. We describe the theory behind our algorithm and briefly discuss the architecture of our software. Finally, we demonstrate both the functionality and utility of software by applying it to two practical examples from videos displaying lymphocyte motility in B cell zones (follicles) and T cell zones of lymph nodes.
影响因子:
56.9
作者:
DENK, W;STRICKLER, JH;WEBB, WW
通讯作者:
WEBB, WW
影响因子:
32.4
作者:
Hauser, Anja E.;Junt, Tobias;Haberman, Ann M.
通讯作者:
Haberman, Ann M.