Dorsal Vagal Complex Modulates Neurogenic Airway Inflammation in a Guinea Pig Model With Esophageal Perfusion of HCl.
Dorsal Vagal Complex Modulates Neurogenic Airway Inflammation in a Guinea Pig Model With Esophageal Perfusion of HCl.
复制标题
背侧迷走神经复合体通过 HCI 食管灌注调节豚鼠模型中的神经源性气道炎症
DOI：
10.3389/fphys.2018.00536
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发表时间：
2018
影响因子：
4
通讯作者：
Lai K
中科院分区：
文献类型：
--
作者：
Chen Z;Sun L;Chen H;Gu D;Zhang W;Yang Z;Peng T;Dong R;Lai K
慢性咳嗽和支气管哮喘中与胃食管反流（格尔）相关的神经源性气道炎症参与了气道-支气管反射，但尚不清楚这种反射是否由中枢神经元介导。本研究旨在探讨豚鼠迷走背侧复合体（DVC）对食管灌流盐酸（HCl）诱导的气道炎症的调节作用。通过测定伊文思蓝染料（EBD）外渗和气道内P物质（SP）表达来评价气道炎症。DVC中的Fos表达指示神经元活性。分别用DiI示踪法和伪狂犬病病毒Bartha（PRV-Bartha）逆行示踪法确定食管下段至DVC和DVC至气道的神经通路。盐酸灌流显著增加血浆外渗，SP在气管中的表达，和SP和Fos在延髓核，包括孤束核（NTS）和迷走神经背侧运动核（DMV）的表达。在DVC内微量注射谷氨酸（Glu）或外源性SP以增强神经元活动，可显著增强食管灌流引起的血浆外渗和SP释放。DVC内微量注射γ-氨基丁酸（GABA）、利多卡因抑制神经元活动或抗SP血清可减轻血浆外渗和SP释放。结论：盐酸食管灌注引起的气道炎症受DVC的调节。本研究为GER相关的气道神经源性炎症机制提供了新的认识。
Neurogenic airway inflammation in chronic cough and bronchial asthma related to gastroesophageal reflux (GER) is involved in the esophageal–bronchial reflex, but it is unclear whether this reflex is mediated by central neurons. This study aimed to investigate the regulatory effects of the dorsal vagal complex (DVC) on airway inflammation induced by the esophageal perfusion of hydrochloric acid (HCl) following the microinjection of nuclei in the DVC in guinea pigs. Airway inflammation was evaluated by measuring the extravasation of Evans blue dye (EBD) and substance P (SP) expression in the airway. Neuronal activity was indicated by Fos expression in the DVC. The neural pathways from the lower esophagus to the DVC and the DVC to the airway were identified using DiI tracing and pseudorabies virus Bartha (PRV-Bartha) retrograde tracing, respectively. HCl perfusion significantly increased plasma extravasation, SP expression in the trachea, and the expression of SP and Fos in the medulla oblongata nuclei, including the nucleus of the solitary tract (NTS) and the dorsal motor nucleus of the vagus (DMV). The microinjection of glutamic acid (Glu) or exogenous SP to enhance neuronal activity in the DVC significantly potentiated plasma extravasation and SP release induced by intra-esophageal perfusion. The microinjection of γ-aminobutyric acid (GABA), lidocaine to inhibit neuronal activity or anti-SP serum in the DVC alleviated plasma extravasation and SP release. In conclusion, airway inflammation induced by the esophageal perfusion of HCl is regulated by DVC. This study provides new insight for the mechanism of airway neurogenic inflammation related to GER.
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DOI：
10.1164/ajrccm/141.3.640
发表时间：
1990-03-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
IRWIN, RS;CURLEY, FJ;FRENCH, CL
通讯作者：
FRENCH, CL
影响因子：
5.9
作者：
Canning, BJ;Mazzone, SB
通讯作者：
Mazzone, SB
DOI：
10.1165/rcmb.2013-0484oc
发表时间：
2014-11-01
影响因子：
6.4
作者：
Cheng, Yan-Mei;Cao, Ai-Li;Wu, Da-Zheng
通讯作者：
Wu, Da-Zheng
影响因子：
24.3
作者：
Daoui, S;D'Agostino, B;Advenier, C
通讯作者：
Advenier, C
影响因子：
9.6
作者：
IRWIN, RS;FRENCH, CL;BENNETT, FM
通讯作者：
BENNETT, FM