Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection
Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection
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人类星形胶质细胞支持柯萨奇病毒 B3 持续感染
DOI：
10.1128/jvi.02090-13
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发表时间：
2013-09
影响因子：
5.4
通讯作者：
Wang, Hanzhong
中科院分区：
文献类型：
--
作者：
Bai, Bingke;Hu, Qinxue;Mao, Panyong;Wang, Hanzhong
摘要肠道病毒经常以人类中枢神经系统为靶点，引起多种神经系统疾病。虽然肠道病毒具有高度的细胞溶解能力，但新出现的证据表明，这些病毒在体内和体外都可以建立持续感染。在这里，我们研究了三种人脑细胞系CCF-STTG1、T98G和SK-N-SH对柯萨奇病毒B3(CVB3)、肠道病毒71和柯萨奇病毒A9三种血清型感染的敏感性。在CVB3感染的CCF-STTG1细胞中观察到持续感染，感染性病毒粒子、病毒RNA和病毒抗原的长期检测证明了这一点。值得注意的是，感染的CCF-STTG1细胞表达了非功能的典型病毒受体柯萨奇病毒-腺病毒受体和衰变加速因子，而从CCF-STTG1细胞中去除细胞表面的硫酸软骨素抑制了CVB3的复制，表明受体的使用是CVB3持续存在的主要限制因素之一。此外，CVB3抑制了感染CCF-STTG1细胞中β干扰素的诱导，这可能是导致持续性的原因之一。此外，在CVB3感染的CCF-STTG1细胞和人类祖细胞来源的星形胶质细胞中，促炎性趋化因子和细胞因子，如血管细胞黏附分子1、白介素8(IL-8)和IL-6被上调。我们的研究结果表明CCF-STTG1细胞有可能成为研究CVB3-中枢神经系统相互作用的一种新的细胞模型，为更好地理解CVB3诱导的慢性神经发病机制提供了基础。
ABSTRACT Enteroviruses can frequently target the human central nervous system to induce a variety of neurological diseases. Although enteroviruses are highly cytolytic, emerging evidence has shown that these viruses can establish persistent infections both in vivo and in vitro. Here, we investigated the susceptibility of three human brain cell lines, CCF-STTG1, T98G, and SK-N-SH, to infection with three enterovirus serotypes: coxsackievirus B3 (CVB3), enterovirus 71, and coxsackievirus A9. Persistent infection was observed in CVB3-infected CCF-STTG1 cells, as evidenced by prolonged detection of infectious virions, viral RNA, and viral antigens. Of note, infected CCF-STTG1 cells expressed the nonfunctional canonical viral receptors coxsackievirus-adenovirus receptor and decay-accelerating factor, while removal of cell surface chondroitin sulfate from CCF-STTG1 cells inhibited the replication of CVB3, suggesting that receptor usage was one of the major limiting factors in CVB3 persistence. In addition, CVB3 curtailed the induction of beta interferon in infected CCF-STTG1 cells, which likely contributed to the initiation of persistence. Furthermore, proinflammatory chemokines and cytokines, such as vascular cell adhesion molecule 1, interleukin-8 (IL-8), and IL-6, were upregulated in CVB3-infected CCF-STTG1 cells and human progenitor-derived astrocytes. Our data together demonstrate the potential of CCF-STTG1 cells to be a novel cell model for studying CVB3-central nervous system interactions, providing the basis toward a better understanding of CVB3-induced chronic neuropathogenesis.
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DOI：
10.1093/infdis/166.5.985
发表时间：
1992-11
期刊：
The Journal of Infectious Diseases
影响因子：
--
作者：
A. Heim;A. Canu;P. Kirschner;T. Simon;G. Mall;P. Hofschneider;R. Kandolf
通讯作者：
A. Heim;A. Canu;P. Kirschner;T. Simon;G. Mall;P. Hofschneider;R. Kandolf
DOI：
10.1111/j.1749-6632.1995.tb27548.x
发表时间：
1995-05
影响因子：
5.2
作者：
P. Muir;F. Nicholson;M. Sharief;E. Thompson;N. Cairns;P. Lantos;G. Spencer;H. Kaminski;J. Banatvala
通讯作者：
P. Muir;F. Nicholson;M. Sharief;E. Thompson;N. Cairns;P. Lantos;G. Spencer;H. Kaminski;J. Banatvala
DOI：
10.4049/jimmunol.171.1.438
发表时间：
2003-07
期刊：
The Journal of Immunology
影响因子：
--
作者：
M. Zanone;E. Favaro;P. Conaldi;J. Greening;A. Bottelli;P. Perin;N. Klein;M. Peakman;G. Camussi
通讯作者：
M. Zanone;E. Favaro;P. Conaldi;J. Greening;A. Bottelli;P. Perin;N. Klein;M. Peakman;G. Camussi
影响因子：
9.9
作者：
Berger, MM;Kopp, N;Lina, B
通讯作者：
Lina, B
DOI：
--
发表时间：
1992
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
A. Heim;A. Canu;P. Kirschner;T. Simon;G. Mall;P. Hofschneider;R. Kandolf
通讯作者：
A. Heim;A. Canu;P. Kirschner;T. Simon;G. Mall;P. Hofschneider;R. Kandolf