Establishment and characterization of a new human extragonadal germ cell line, SEM-1, and its comparison with TCam-2 and JKT-1.
Establishment and characterization of a new human extragonadal germ cell line, SEM-1, and its comparison with TCam-2 and JKT-1.
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DOI：
10.1016/j.urology.2012.09.029
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发表时间：
2013-02
期刊：
影响因子：
2.1
通讯作者：
Epstein AL
中科院分区：
文献类型：
--
作者：
Russell SM;Lechner MG;Mokashi A;Megiel C;Jang JK;Taylor CR;Looijenga LH;French CA;Epstein AL
描述一个人细胞系SEM-1的建立和特征，该细胞系来自一个诊断为纵隔腺瘤的患者。一小部分生殖细胞肿瘤发生于性腺外部位，其病因学尚不清楚。目前，仅报道了2种来自睾丸癌患者的细胞系，JKT-1和TCam-2，两者均来源于睾丸。通过裸鼠异种移植、细胞遗传学研究、生殖细胞肿瘤生物标志物的免疫组化和流式细胞术染色、癌症睾丸抗原表达的定量逆转录聚合酶链反应以及定量聚合酶链反应的BRAF突变筛查对细胞系进行表征。表征研究证实了SEM-1的人性腺外腺瘤起源，并证明它与TCam-2比JKT-1具有更多的共同特征。具体而言，SEM-1对Sal样蛋白4（萨尔-4）、激活蛋白-2 γ（AP-2γ）和细胞角蛋白CAM 5.2呈阳性，并且证明干细胞标志物八聚体结合转录因子3/4、NANOG、c-KIT、SOX 17和SOX 2的异质表达。细胞遗传学分析显示亚三倍体染色体数目，12 p的多个拷贝，但等染色体12 p和BRAF突变V600 E未被确定。这些细胞系也不含中线癌中观察到的BRD 4/NUT基因重排[t（15，19）]，也不含睾丸核蛋白（NUT）基因过表达。SEM-1是第一个来源于性腺外生殖细胞肿瘤的细胞系，表现出介于腺瘤和非腺瘤之间的中间特征，因此是研究这种恶性肿瘤分子发病机制的重要模型。
To describe the establishment and characterization of a human cell line, SEM-1, from a patient diagnosed with a mediastinal seminoma. A small percentage of germ cell tumors develop as primary lesions in extragonadal sites, and the etiology of these tumors is poorly understood. Currently, only 2 cell lines from seminoma patients have been reported, JKT-1 and TCam-2, both derived from the testis. The cell line was characterized by heterotransplantation in Nude mice, cytogenetic studies, immunohistochemical and flow cytometry staining for germ cell tumor biomarkers, quantitative reverse-transcription polymerase chain reaction for cancer testis antigen expression, and BRAF mutation screening with quantitative polymerase chain reaction. Characterization studies confirmed the human extragonadal seminoma origin of SEM-1 and demonstrated that it had more features in common with TCam-2 than JKT-1. Specifically, SEM-1 was positive for Sal-like protein 4 (SALL-4), activator protein-2γ (AP-2γ), and cytokeratin CAM5.2, and demonstrated heterogeneous expression of stem cell markers octamer-binding transcription factor 3/4, NANOG, c-KIT, SOX17, and SOX2. Cytogenetic analysis revealed a hypotriploid chromosome number, with multiple copies of 12p, but isochromosome 12p and the BRAF mutation V600E were not identified. The cell lines also did not contain the BRD4/NUT gene rearrangement [t(15,19)] seen in midline carcinomas nor did they contain overexpressed nuclear protein in testis (NUT) genes. SEM-1 is the first cell line derived from an extragonadal germ cell tumor showing intermediate characteristics between seminoma and nonseminoma, and as such, is an important model to study the molecular pathogenesis of this malignancy.
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影响因子：
6.4
作者：
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通讯作者：
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影响因子：
4.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Schwartz, S
DOI：
10.1097/00129039-200609000-00004
发表时间：
2006-09-01
影响因子：
1.6
作者：
Jung, Shih-Ming;Chu, Pao-Hsien;Lin, Pyng-Jing
通讯作者：
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