Ascorbic acid analysis in biological fluids.

Ascorbic acid analysis in biological fluids.
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生物体液中的抗坏血酸分析。

DOI:
10.1016/0003-2697(82)90462-6
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发表时间:
1982
影响因子:
2.9
通讯作者:
Nahrwold,DL
Nahrwold,DL
中科院分区:
生物学4区
文献类型:
--
作者:
Rose,RC;Nahrwold,DL

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介绍了高压液相色谱法在生物样品中抗坏血酸定量分析中的应用。选择的实验条件可能代表在广泛的研究和临床试验下满足的条件。该技术被认为是提供的抗坏血酸在豚鼠组织的各种样品中的值与现有的分析方法一致。分析所需的时间短,并且在临床和研究实验室中越来越频繁地使用实验仪器,这使得这种方法具有吸引力。在不同的温度和pH值的抗坏血酸的衰减率进行了评估。报告了禁食和非禁食豚鼠几种组织中抗坏血酸的内源性水平。
The application of high-pressure liquid chromatography to quantitative analysis of ascorbic acid in biological samples is described. Experimental conditions were selected that might be representative of those met under a broad range of research and clinical trials. The technique is seen to provide values of ascorbic acid in various samples of guinea pig tissues that are in agreement with an existing analytical method. The short time required for analysis and the use of experimental apparatus that is found with increasing frequency in clinical and research laboratories make this approach attractive. The decay rates of ascorbic acid at different values of temperature and pH are evaluated. Endogenous levels of ascorbic acid in several tissues of fasted and nonfasted guinea pigs are reported.
DOI: 10.1085/jgp.49.5.849
发表时间: 1966-05
期刊: The Journal of general physiology
影响因子: --
作者:
Schultz SG;Fuisz RE;Curran PF
通讯作者: Curran PF
DOI: 10.1021/ac60366a045
发表时间: 1976-01-01
影响因子: 7.4
作者:
PACHLA, LA;KISSINGER, PT
通讯作者: KISSINGER, PT
DOI: 10.1042/bj0620675
发表时间: 1956
期刊: The Biochemical journal
影响因子: --
作者:
J. Owen;B. Iggo
通讯作者: B. Iggo
采用高效液相色谱法定量分析抗坏血酸和脱氢抗坏血酸。
DOI: 10.1016/0003-2697(81)90464-4
发表时间: 1981
影响因子: 2.9
作者:
Rose,RC;Nahrwold,DL
通讯作者: Nahrwold,DL