The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation.
The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation.
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DOI：
10.1038/s41598-017-06969-4
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发表时间：
2017-07-31
影响因子：
4.6
通讯作者：
Nakazawa T
中科院分区：
文献类型：
--
作者：
Maekawa S;Sato K;Fujita K;Daigaku R;Tawarayama H;Murayama N;Moritoh S;Yabana T;Shiga Y;Omodaka K;Maruyama K;Nishiguchi KM;Nakazawa T
我们在氧化应激下的原代视网膜细胞培养物中筛选了 41 种材料的抗氧化特性后，发现橙皮苷（一种植物来源的生物类黄酮）可能是视网膜神经保护治疗的候选药物。我们发现，向小鼠玻璃体内注射橙皮苷可防止 N-甲基-D-天冬氨酸 (NMDA) 诱导的兴奋性毒性后视网膜神经节细胞 (RGC) 标记物的减少和 RGC 死亡。橙皮苷治疗还减少了钙蛋白酶的激活、活性氧的产生和 TNF-α 基因的表达。最后，橙皮苷治疗改善了电生理功能（通过视觉诱发电位测量）和视觉功能（通过验光测量）。因此，我们发现橙皮苷抑制了许多与 NMDA 诱导的细胞死亡信号相关的细胞毒性因子，如氧化应激、钙蛋白酶过度激活和炎症，从而保护小鼠的 RGC。因此，橙皮苷可能具有作为治疗补充剂的潜力，用于保护视网膜免受与兴奋性毒性损伤相关的损伤，例如青光眼和糖尿病性视网膜病变中发生的损伤。
We found that hesperidin, a plant-derived bioflavonoid, may be a candidate agent for neuroprotective treatment in the retina, after screening 41 materials for anti-oxidative properties in a primary retinal cell culture under oxidative stress. We found that the intravitreal injection of hesperidin in mice prevented reductions in markers of the retinal ganglion cells (RGCs) and RGC death after N-methyl-D-aspartate (NMDA)-induced excitotoxicity. Hesperidin treatment also reduced calpain activation, reactive oxygen species generation and TNF-α gene expression. Finally, hesperidin treatment improved electrophysiological function, measured with visual evoked potential, and visual function, measured with optomotry. Thus, we found that hesperidin suppressed a number of cytotoxic factors associated with NMDA-induced cell death signaling, such as oxidative stress, over-activation of calpain, and inflammation, thereby protecting the RGCs in mice. Therefore, hesperidin may have potential as a therapeutic supplement for protecting the retina against the damage associated with excitotoxic injury, such as occurs in glaucoma and diabetic retinopathy.
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影响因子：
15.9
作者：
Harada, Takayuki;Harada, Chikako;Tanaka, Kohichi
通讯作者：
Tanaka, Kohichi
影响因子：
4.7
作者：
Kanamori, Akiyasu;Catrinescu, Maria-Magdalena;Levin, Leonard A.
通讯作者：
Levin, Leonard A.
DOI：
10.1002/neu.480230915
发表时间：
1992-11-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
CHOI, DW
通讯作者：
CHOI, DW
影响因子：
--
作者：
Kassoff, A;Kassoff, J;Chew, EY
通讯作者：
Chew, EY
影响因子：
9.9
作者：
Gao, X.;Cassidy, A.;Ascherio, A.
通讯作者：
Ascherio, A.