SiLEA14, a novel atypical LEA protein, confers abiotic stress resistance in foxtail millet.
SiLEA14, a novel atypical LEA protein, confers abiotic stress resistance in foxtail millet.
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DOI：
10.1186/s12870-014-0290-7
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发表时间：
2014-11-18
影响因子：
5.3
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Wang M;Li P;Li C;Pan Y;Jiang X;Zhu D;Zhao Q;Yu J
晚期胚胎发生丰富（LEA）蛋白参与保护高等植物免受环境胁迫造成的损害。谷子（Setaria italica）是半干旱地区重要的粮食和饲料谷类作物。然而，对这些条件耐受的分子机制尚未明确。在这里，我们从谷子中鉴定了一种名为 SiLEA14 的新型非典型 LEA 基因。它包含由一个内含子分隔的两个外显子。在正常生长条件下，SiLEA14在根、茎、叶、花序和种子中均有不同水平的表达。此外，渗透胁迫、氯化钠和外源脱落酸显着诱导 SiLEA14。 SiLEA14蛋白定位于细胞核和细胞质。与对照相比，SiLEA14 在盐胁迫下的过度表达改善了大肠杆菌的生长性能。为了进一步评估SiLEA14在植物中的功能，获得了过度表达SiLEA14的转基因拟南芥和谷子植物。转基因拟南芥幼苗比野生型（WT）表现出更高的盐和渗透胁迫耐受性。同样，与野生型相比，转基因谷子在盐和干旱胁迫下表现出更好的生长。综上所述，我们的结果表明 SiLEA14 是一种新型非典型 LEA 蛋白，在植物抵抗非生物胁迫方面发挥着重要作用。我们从谷子中鉴定了一种新型非典型 LEA 基因 SiLEA14，该基因在植物非生物胁迫抗性中发挥重要作用。 SiLEA14 表达的修饰可能会提高农作物的非生物胁迫抗性。本文的在线版本 (doi:10.1186/s12870-014-0290-7) 包含补充材料，可供授权用户使用。
Late embryogenesis abundant (LEA) proteins are involved in protecting higher plants from damage caused by environmental stresses. Foxtail millet (Setaria italica) is an important cereal crop for food and feed in semi-arid areas. However, the molecular mechanisms underlying tolerance to these conditions are not well defined. Here, we characterized a novel atypical LEA gene named SiLEA14 from foxtail millet. It contains two exons separated by one intron. SiLEA14 was expressed in roots, stems, leaves, inflorescences and seeds at different levels under normal growth conditions. In addition, SiLEA14 was dramatically induced by osmotic stress, NaCl and exogenous abscisic acid. The SiLEA14 protein was localized in the nucleus and the cytoplasm. Overexpression of SiLEA14 improved Escherichia coli growth performance compared with the control under salt stress. To further assess the function of SiLEA14 in plants, transgenic Arabidopsis and foxtail millet plants that overexpressed SiLEA14 were obtained. The transgenic Arabidopsis seedlings showed higher tolerance to salt and osmotic stress than the wild type (WT). Similarly, the transgenic foxtail millet showed improved growth under salt and drought stresses compared with the WT. Taken together, our results indicated that SiLEA14 is a novel atypical LEA protein and plays important roles in resistance to abiotic stresses in plants. We characterized a novel atypical LEA gene SiLEA14 from foxtail millet, which plays important roles in plant abiotic stress resistance. Modification of SiLEA14 expression may improve abiotic stress resistance in agricultural crops. The online version of this article (doi:10.1186/s12870-014-0290-7) contains supplementary material, which is available to authorized users.
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