OsLEA3-2, an abiotic stress induced gene of rice plays a key role in salt and drought tolerance.
OsLEA3-2, an abiotic stress induced gene of rice plays a key role in salt and drought tolerance.
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水稻非生物胁迫诱导基因 OsLEA3-2 在耐盐耐旱中发挥关键作用
DOI：
10.1371/journal.pone.0045117
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Cai W
中科院分区：
文献类型：
--
作者：
Duan J;Cai W
晚期胚胎发生丰富(LEA)蛋白参与了许多不同生物对干旱、寒冷和高盐的耐受性。利用快速扩增基因末端扩增(RACE)技术，在水稻中鉴定到一个LEA蛋白产生全长基因OsLEA3-2。OsLEA3-2仅在胚胎中表达，并可被非生物胁迫诱导。编码蛋白定位于细胞核，OsLEA3-2在酵母中的过表达改善了酵母在盐和渗透胁迫条件下的生长性能。OsLEA3-2也被插入到PHB载体中，并在拟南芥和水稻中高效表达。转基因拟南芥幼苗在附加150 mM甘露醇或100 mM NaC l的MS培养基上的生长表现优于野生型植株。转基因水稻在盐胁迫和渗透胁迫条件下的生长表现也明显强于对照，在干旱胁迫20天后能够恢复。体外分析表明，OsLEA3-2能够保护LDH在冷冻过程中不聚集，在干燥过程中失活。这些结果表明，OsLEA3-2在植物对非生物胁迫的耐受性中起重要作用。
Late embryogenesis abundant (LEA) proteins are involved in tolerance to drought, cold and high salinity in many different organisms. In this report, a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa) using the Rapid Amplification of cDNA Ends (RACE) method. OsLEA3-2 was found to be only expressed in the embryo and can be induced by abiotic stresses. The coding protein localizes to the nucleus and overexpression of OsLEA3-2 in yeast improved growth performance compared with control under salt- and osmotic-stress conditions. OsLEA3-2 was also inserted into pHB vector and overexpressed in Arabidopsis and rice. The transgenic Arabidopsis seedlings showed better growth on MS media supplemented with 150 mM mannitol or 100 mM NaCl as compared with wild type plants. The transgenic rice also showed significantly stronger growth performance than control under salinity or osmotic stress conditions and were able to recover after 20 days of drought stress. In vitro analysis showed that OsLEA3-2 was able to protect LDH from aggregation on freezing and inactivation on desiccation. These results indicated that OsLEA3-2 plays an important role in tolerance to abiotic stresses.
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影响因子：
3
作者：
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通讯作者：
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影响因子：
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通讯作者：
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作者：
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