Proteomic analysis of soybean defense response induced by cotton worm (prodenia litura, fabricius) feeding.
Proteomic analysis of soybean defense response induced by cotton worm (prodenia litura, fabricius) feeding.
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棉虫（prodenia litura，fabricius）取食诱导的大豆防御反应的蛋白质组学分析
DOI：
10.1186/1477-5956-10-16
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发表时间：
2012-03-08
期刊：
影响因子：
2
通讯作者：
Yu D
中科院分区：
文献类型：
--
作者：
Fan R;Wang H;Wang Y;Yu D
研究背景棉虫是我国南方地区大豆的主要害虫之一。植物可能会获得防御机制，以免受食草动物的捕食。诱导反应可以导致许多物种对食草动物的抵抗力增强。本研究的重点是寻找棉虫摄食诱导的大豆防御反应中发生变化的蛋白质。结果通过二维凝胶电泳（2-DE）鉴定出10个因棉虫摄食而发生丰度变化的蛋白质点。通过 MALDI-TOF MS 鉴定出这些点中总共 11 种独特的蛋白质。大多数变化蛋白的 mRNA 和蛋白相对表达水平上调。这些蛋白主要参与活性氧清除、防御信号转导和代谢调节等生理过程。结论这是首次对棉虫诱导的大豆防御反应进行蛋白质组学分析。差异表达的蛋白质可以共同作用，在诱导的防御反应中发挥重要作用。 PAL 和 SAMS 在蛋白质和 mRNA 水平上均上调。这些基因可以成为进一步功能研究的最强候选基因。
BackgroundCotton worm is one of the main insects of soybean in southern China. Plants may acquire defense mechanisms that confer protection from predation by herbivores. Induced responses can lead to increased resistance against herbivores in many species. This study focuses on searching changed proteins in soybean defense response induced by cotton worm feeding.ResultsTen protein spots that are changed in abundance in response to cotton worm feeding were identified by Two-dimensional gel electrophoresis (2-DE). A total of 11 unique proteins from these spots were identified by MALDI-TOF MS. The mRNA and protein relative expression levels of most changed proteins were up-regulated. These proteins were mainly involved in physiological processes, including active oxygen removal, defense signal transduction, and metabolism regulation.ConclusionThis is the first proteomic analysis of the soybean defense response induced by cotton worm. The differentially expressed proteins could work together to play a major role in the induced defense response. PAL and SAMS were up-regulated at both the protein and mRNA levels. These genes can be strongest candidates for further functional research.
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影响因子：
7
作者：
Beyer, A;Hollunder, J;Wilhelm, T
通讯作者：
Wilhelm, T
影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
GROSS, HJ
影响因子：
56.9
作者：
Agrawal, AA
通讯作者：
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影响因子：
2.7
作者：
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通讯作者：
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影响因子：
5.1
作者：
ESPARTERO, J;PINTORTORO, JA;PARDO, JM
通讯作者：
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