Sonic hedgehog-dependent recruitment of GABAergic interneurons into the developing visual thalamus.
Sonic hedgehog-dependent recruitment of GABAergic interneurons into the developing visual thalamus.
复制标题
作者：
Somaiya RD;Stebbins K;Gingrich EC;Xie H;Campbell JN;Garcia ADR;Fox MA
视网膜神经节细胞（RGCs）轴突在视丘脑抑制回路的发育中起着重要作用。我们以前报道，RGC轴突信号星形胶质细胞诱导成纤维细胞生长因子15（FGF15）的表达，GABA能中间神经元迁移到视丘脑所需的原。然而，视网膜轴突如何诱导丘脑星形胶质细胞产生Fgf15并影响神经元间迁移仍不清楚。在这里，我们证明，削弱RGC活动的影响不大，对小鼠视觉丘脑的中间神经元招聘。相反，我们的数据表明，视网膜衍生的音刺猬（SHH）是必不可少的interneuron招聘。具体来说，我们发现丘脑投射RGCs表达SHH和丘脑星形胶质细胞产生SHH信号的下游组件。RGC衍生的SHH的缺失导致FGF15表达的显著降低，以及招募到视觉丘脑中的中间神经元的百分比。总的来说，我们的研究结果确定了一个形态依赖性的神经元星形胶质细胞的信号传导机制至关重要的丘脑中间神经元的迁移。
Axons of retinal ganglion cells (RGCs) play critical roles in the development of inhibitory circuits in visual thalamus. We previously reported that RGC axons signal astrocytes to induce the expression of fibroblast growth factor 15 (FGF15), a motogen required for GABAergic interneuron migration into visual thalamus. However, how retinal axons induce thalamic astrocytes to generate Fgf15 and influence interneuron migration remains unknown. Here, we demonstrate that impairing RGC activity had little impact on interneuron recruitment into mouse visual thalamus. Instead, our data show that retinal-derived sonic hedgehog (SHH) is essential for interneuron recruitment. Specifically, we show that thalamus-projecting RGCs express SHH and thalamic astrocytes generate downstream components of SHH signaling. Deletion of RGC-derived SHH leads to a significant decrease in Fgf15 expression, as well as in the percentage of interneurons recruited into visual thalamus. Overall, our findings identify a morphogen-dependent neuron–astrocyte signaling mechanism essential for the migration of thalamic interneurons.
DOI：
10.1002/cne.25261
发表时间：
2022-05
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Somaiya RD;Huebschman NA;Chaunsali L;Sabbagh U;Carrillo GL;Tewari BP;Fox MA
通讯作者：
Fox MA