Development of astrocyte morphology and function in mouse visual thalamus.
Development of astrocyte morphology and function in mouse visual thalamus.
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DOI：
10.1002/cne.25261
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发表时间：
2022-05
期刊：
影响因子：
--
通讯作者：
Fox MA
中科院分区：
文献类型：
--
作者：
Somaiya RD;Huebschman NA;Chaunsali L;Sabbagh U;Carrillo GL;Tewari BP;Fox MA
啮齿类动物视丘已成为阐明感觉回路形成和功能的细胞和分子机制的有力模型。尽管我们对轴突-靶相互作用和神经活动在视觉丘脑中协调电路形成的作用的理解取得了重大进展，但非神经元细胞（如星形胶质细胞）的作用尚不清楚。事实上，我们对小鼠视丘脑星形胶质细胞的转录特性和发育知之甚少。为了解决这一知识的差距，我们研究了典型的星形胶质细胞分子在视觉丘脑的表达，免疫染色，原位杂交和报告线。虽然我们的数据表明，在视觉丘脑的不同核的星形胶质细胞的异质性的一些水平，大多数丘脑星形胶质细胞出现标记在Aldh 1 l1-EGFP小鼠。这使得我们使用这个转基因系来表征这些细胞在视觉丘脑中的新生儿和出生后发育。我们的数据表明，不仅有整个队列的星形胶质细胞迁移到视觉丘脑的眼睛睁开，但他们也获得了他们的成年样形态，即使视网膜神经突触仍在成熟。此外，超微结构，免疫组织化学和功能的方法表明，通过睁眼，丘脑星形胶质细胞包鞘retinogeniculate突触，并能够有效地摄取谷氨酸。两者合计，我们的研究结果表明，视觉丘脑星形胶质细胞的形态，解剖和功能的发展发生在睁眼和经验依赖性视觉活动的出现之前。图形摘要文本。视丘脑星形胶质细胞的形态和功能发育在本研究中，我们发现，通过睁眼，星形胶质细胞不仅具有成人样的形态，而且完全包裹视网膜神经突触，并有效地摄取细胞外谷氨酸
The rodent visual thalamus has served as a powerful model to elucidate the cellular and molecular mechanisms that underlie sensory circuit formation and function. Despite significant advances in our understanding of the role of axon-target interactions and neural activity in orchestrating circuit formation in visual thalamus, the role of non-neuronal cells, such as astrocytes, is less clear. In fact, we know little about the transcriptional identity and development of astrocytes in mouse visual thalamus. To address this gap in knowledge, we studied the expression of canonical astrocyte molecules in visual thalamus using immunostaining, in situ hybridization, and reporter lines. While our data suggests some level of heterogeneity of astrocytes in different nuclei of the visual thalamus, the majority of thalamic astrocytes appeared to be labelled in Aldh1l1-EGFP mice. This led us to use this transgenic line to characterize the neonatal and postnatal development of these cells in visual thalamus. Our data show that not only have the entire cohort of astrocytes migrated into visual thalamus by eye-opening but they also have acquired their adult-like morphology, even while retinogeniculate synapses are still maturing. Furthermore, ultrastructural, immunohistochemical, and functional approaches revealed that by eye-opening, thalamic astrocytes ensheath retinogeniculate synapses and are capable of efficient uptake of glutamate. Taken together, our results reveal that the morphological, anatomical, and functional development of astrocytes in visual thalamus occurs prior to eye-opening and the emergence of experience-dependent visual activity. Graphical Abstract Text. Morphological and functional development of astrocytes in the visual thalamus In the present study, we show that by eye-opening, astrocytes not only possess adult-like morphology but also completely enwrap retinogeniculate synapses and efficiently uptake extracellular glutamate
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DOI：
10.1002/cne.22223
发表时间：
2010-03-01
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Bickford ME;Slusarczyk A;Dilger EK;Krahe TE;Kucuk C;Guido W
通讯作者：
Guido W
影响因子：
2.7
作者：
Agius, E;Soukkarieh, C;Cochard, P
通讯作者：
Cochard, P
影响因子：
2.5
作者：
GODEMENT, P;SALAUN, J;IMBERT, M
通讯作者：
IMBERT, M
影响因子：
2.5
作者：
Fu, Yuhong;Tvrdik, Petr;Watson, Charles
通讯作者：
Watson, Charles
通过神经胶质调节突触连通性。
影响因子：
64.8
作者：
Eroglu, Cagla;Barres, Ben A.
通讯作者：
Barres, Ben A.