Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron.
Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron.
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DOI：
10.1186/s12985-022-01827-w
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发表时间：
2022-05-28
期刊：
影响因子：
4.8
通讯作者：
Zhang, Zheng
中科院分区：
文献类型：
--
作者：
Zhou, Bing;Cheng, Lin;Song, Shuo;Guo, Huimin;Shen, Senlin;Wang, Haiyan;Ge, Xiangyang;Liu, Lei;Ju, Bin;Zhang, Zheng
The SARS-CoV-2 nucleocapsid protein (NP) is an important indicator for the virus infection, highlighting the crucial role of NP-specific monoclonal antibodies (mAbs) used in multiple biochemical assays and clinical diagnosis for detecting the NP antigen. Here, we reported a pair of noncompeting human NP-specific mAbs, named P301-F7 and P301-H5, targeting two distinct linear epitopes on SARS-CoV-2 or SARS-CoV. We evaluated the application of P301-F7 in the analysis of enzyme linked immunosorbent assay, western blot, flow cytometry, immunofluorescence, and focus reduction neutralization test. We for the first time report a broad mAb effectively recognizing various live viruses of SARS-CoV-2 variants including Alpha, Beta, Delta, and Omicron, indicating a wide range of application prospects. The online version contains supplementary material available at 10.1186/s12985-022-01827-w.
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影响因子：
12.7
作者：
Xie, Chengzuo;Ding, Haojie;Ding, Jianzu;Xue, Yangji;Lu, Shaohong;Lv, Hangjun
通讯作者：
Lv, Hangjun
DOI：
10.1016/j.xcrm.2021.100311
发表时间：
2021-06-15
期刊：
Cell reports. Medicine
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作者：
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通讯作者：
Ryo A
影响因子：
64.5
作者：
Liu C;Ginn HM;Dejnirattisai W;Supasa P;Wang B;Tuekprakhon A;Nutalai R;Zhou D;Mentzer AJ;Zhao Y;Duyvesteyn HME;López-Camacho C;Slon-Campos J;Walter TS;Skelly D;Johnson SA;Ritter TG;Mason C;Costa Clemens SA;Gomes Naveca F;Nascimento V;Nascimento F;Fernandes da Costa C;Resende PC;Pauvolid-Correa A;Siqueira MM;Dold C;Temperton N;Dong T;Pollard AJ;Knight JC;Crook D;Lambe T;Clutterbuck E;Bibi S;Flaxman A;Bittaye M;Belij-Rammerstorfer S;Gilbert SC;Malik T;Carroll MW;Klenerman P;Barnes E;Dunachie SJ;Baillie V;Serafin N;Ditse Z;Da Silva K;Paterson NG;Williams MA;Hall DR;Madhi S;Nunes MC;Goulder P;Fry EE;Mongkolsapaya J;Ren J;Stuart DI;Screaton GR
通讯作者：
Screaton GR
DOI：
10.1016/j.jbc.2021.101290
发表时间：
2022-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gransagne M;Aymé G;Brier S;Chauveau-Le Friec G;Meriaux V;Nowakowski M;Dejardin F;Levallois S;Dias de Melo G;Donati F;Prot M;Brûlé S;Raynal B;Bellalou J;Goncalves P;Montagutelli X;Di Santo JP;Lazarini F;England P;Petres S;Escriou N;Lafaye P
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Lafaye P
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影响因子：
16.6
作者：
Kang S;Yang M;He S;Wang Y;Chen X;Chen YQ;Hong Z;Liu J;Jiang G;Chen Q;Zhou Z;Zhou Z;Huang Z;Huang X;He H;Zheng W;Liao HX;Xiao F;Shan H;Chen S
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Chen S