Characterization of local fluid flow in 3D porous construct characterized by Fourier domain Doppler optical coherence tomography
Characterization of local fluid flow in 3D porous construct characterized by Fourier domain Doppler optical coherence tomography
复制标题
通过傅里叶域多普勒光学相干断层扫描表征 3D 多孔结构中的局部流体流动
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
R. Wang
中科院分区：
文献类型：
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作者：
P. Bagnaninchi;Y. Yang;A. E. El Haj;M. Hinds;R. Wang
为了获得具有正确生物力学特性的功能组织，机械刺激细胞是至关重要的。灌注生物反应器诱导的流体剪切应力已被充分表征为二维培养，其中模拟和实验数据都是可用的。然而，这些结果不能直接转化为组织工程，利用复杂的三维多孔支架。此外，受刺激的细胞产生大量的细胞外基质（ECM），其显著改变了构建体的微结构，改变了局部流动动力学。在这项研究中，傅立叶域多普勒光学相干层析成像（FD-DOCT）系统工作在1300 nm的带宽为50 nm已被用来确定内部的不同类型的多孔支架用于组织工程中的局部流速。然后，对于牛顿流体，局部流速可以与在孔壁上发生的流体剪切应力线性相关。多孔壳聚糖支架（1.5mm × 3 mm），具有和没有一个中央250米的微通道已通过冷冻干燥技术生产。这种技术使我们能够确定施加到细胞的实际剪切应力，并因此优化输入流速，而且还将流量分布的变化与ECM产生的量相关联，从而允许监测组织形成。
In order to achieve functional tissue with the correct biomechanical properties it is critical to stimulate mechanically the cells. Perfusion bioreactor induces fluid shear stress that has been well characterized for two-dimensional culture where both simulation and experimental data are available. However these results can't be directly translated to tissue engineering that makes use of complex three-dimensional porous scaffold. Moreover, stimulated cells produce extensive extra-cellular matrix (ECM) that alter dramatically the micro-architecture of the constructs, changing the local flow dynamic. In this study a Fourier domain Doppler optical coherent tomography (FD-DOCT) system working at 1300nm with a bandwidth of 50nm has been used to determine the local flow rate inside different types of porous scaffolds used in tissue engineering. Local flow rates can then be linearly related, for Newtonian fluid, to the fluid shear stress occurring on the pores wall. Porous chitosan scaffolds (&fgr;1.5mm x 3mm) with and without a central 250 &mgr;m microchannel have been produced by a freeze-drying technique. This techniques allow us to determine the actual shear stress applied to the cells and to optimise the input flow rate consequently, but also to relate the change of the flow distribution to the amount of ECM production allowing the monitoring of tissue formation.
影响因子：
14
作者：
Goldstein, AS;Juarez, TM;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
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作者：
Davisson, T;Sah, RL;Ratcliffe, A
通讯作者：
Ratcliffe, A