Developing a prognostic micro-RNA signature for human cervical carcinoma.
Developing a prognostic micro-RNA signature for human cervical carcinoma.
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DOI：
10.1371/journal.pone.0123946
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Liu FF
中科院分区：
文献类型：
--
作者：
How C;Pintilie M;Bruce JP;Hui AB;Clarke BA;Wong P;Yin S;Yan R;Waggott D;Boutros PC;Fyles A;Hedley DW;Hill RP;Milosevic M;Liu FF
宫颈癌仍然是全球女性第三大最常诊断的癌症和第四大癌症死亡原因。我们试图开发一种可预测无病生存的 micro-RNA 特征，从而有可能为宫颈癌患者量身定制治疗方案。在 79 个冷冻样本的训练集中确定了候选预后 9-micro-RNA 特征集。然而，在由 87 名福尔马林固定石蜡包埋 (FFPE) 标本组成的独立队列中，采用三种不同的方法验证这一特征，但并未成功。开发宫颈癌预后 micro-RNA 特征存在一些挑战和考虑因素，即：肿瘤异质性、冷冻和 FFPE 标本之间缺乏一致性，以及该疾病的整体 micro-RNA 表达谱的平台选择。我们的观察结果为未来宫颈癌的 miRNA 特征研究提供了重要的警示，这也可能适用于涉及其他类型人类恶性肿瘤的 miRNA 分析研究。
Cervical cancer remains the third most frequently diagnosed and fourth leading cause of cancer death in women worldwide. We sought to develop a micro-RNA signature that was prognostic for disease-free survival, which could potentially allow tailoring of treatment for cervical cancer patients. A candidate prognostic 9-micro-RNA signature set was identified in the training set of 79 frozen specimens. However, three different approaches to validate this signature in an independent cohort of 87 patients with formalin-fixed paraffin-embedded (FFPE) specimens, were unsuccessful. There are several challenges and considerations associated with developing a prognostic micro-RNA signature for cervical cancer, namely: tumour heterogeneity, lack of concordance between frozen and FFPE specimens, and platform selection for global micro-RNA expression profiling in this disease. Our observations provide an important cautionary tale for future miRNA signature studies for cervical cancer, which can also be potentially applicable to miRNA profiling studies involving other types of human malignancies.
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影响因子：
5
作者：
Hui, Angela B. Y.;Shi, Wei;Liu, Fei-Fei
通讯作者：
Liu, Fei-Fei
影响因子：
45.3
作者：
Fyles, A;Milosevic, M;Hill, RP
通讯作者：
Hill, RP
DOI：
10.1097/00004347-199410000-00006
发表时间：
1994-10-01
影响因子：
2.4
作者：
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通讯作者：
KIRK, ME
影响因子：
6.5
作者：
Glud, Martin;Klausen, Mikkel;Drzewiecki, Krzysztof T.
通讯作者：
Drzewiecki, Krzysztof T.
影响因子：
14.9
作者：
Griffiths-Jones S;Grocock RJ;van Dongen S;Bateman A;Enright AJ
通讯作者：
Enright AJ