Molecular phylogeny and pathogenicity of indigenous Beauveria bassiana against the tomato leafminer, Tuta absoluta Meyrick 1917 (Lepidoptera: Gelechiidae), in Ethiopia.
Molecular phylogeny and pathogenicity of indigenous Beauveria bassiana against the tomato leafminer, Tuta absoluta Meyrick 1917 (Lepidoptera: Gelechiidae), in Ethiopia.
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DOI：
10.1186/s43141-021-00227-x
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发表时间：
2021-08-26
期刊：
影响因子：
--
通讯作者：
Assefa F
中科院分区：
文献类型：
--
作者：
Aynalem B;Muleta D;Venegas J;Assefa F
Tuta absoluta Meyrick 1917（鳞翅目：麦蛾科）是一种入侵性、抗药性强的害虫，是世界番茄生产上的主要害虫。它需要有效的管理选择，自然感染昆虫，而不会引起任何确定的副作用。昆虫病原真菌（EPF）是最重要的选择。然而，地理来源和气候条件显然造成EPF菌株之间的遗传变异，影响其致病性。因此，从地方资源中筛选出有效的EPF菌株，对发展环境友好的防治策略具有重要意义。绝对的从不同类型土壤中分离到27株白僵菌，并对其中12株白僵菌进行了生物学效率指数（BEI）筛选。对2龄和3龄幼虫的致死率分别为65.7-95.7%和68.3-95%。接种后7 d，在1 × 107孢子·ml-1的浓度下培养，无菌生长。其中，5个菌株（18.5%）对两龄幼虫的致死率均在90%以上，孢子浓度最低（104孢子·ml-1）时的LT 50值为3.33 ~ 5.33 d，最高（108孢子·ml-1）时的LT 50值为1.93 ~ 3.17 d，LC 50值为1.5 × 103 ~ 1.1× 105孢子·ml-1。此外，菌株的死亡率较高（1.5 × 106 ~ 3.5 × 107个孢子·ml-1），能在幼虫尸体上产孢，在最适温度下生长良好，并能产生几丁质降解酶。分子生物学分析表明，分离物具有近单系特征，并归类于球孢白僵菌种下。埃塞俄比亚不同类型的土壤是B的重要来源。这些菌株对白僵菌具有较好的致病性。absoluta，这对于生态友好型生物农药的开发至关重要。虽然在系统发育研究中，分离物几乎是单系的，但其中5个分离物在实验室生物测定中对T。绝对值;然而，大规模生产需要进一步的现场评估。
Tuta absoluta Meyrick 1917 (Lepidoptera: Gelechiidae) is an invasive, pesticide resistant, and a major treat of tomato production in the world. It needs effective management options that naturally infect the insect without causing any identified side effects. Entomopathogenic fungi (EPF) are the most important options. However, geographic origin and climatic condition apparently creates genetic variation among EPF strains that influence on their pathogenicity. Thus, screening of effective EPF strains from the local source is vital to develop environmental friendly pest control tactic for T. absoluta. In this study, 27 indigenous Beauveria were isolated from the various types of soil and 12 of the isolates were screened based on their biological efficiency index (BEI). These isolates scored 65.7–95.7% and 68.3–95% of mortality against second and third instar larvae of T. absoluta at concentration of 1 × 107spores·ml-1 in 7 days post inoculation, respectively. Out of these, five (18.5%) isolates scored above 90% mortality on both instar larvae with LT50 value of 3.33 to 5.33 days at the lowest (104 spores·ml-1) and 1.93 to 3.17 days at highest (108 spores·ml-1) spore concentrations and has LC50 value of 1.5 × 103 to 1.1× 105 spores·ml-1. Moreover, isolates exhibited the promising mortality better (1.5 × 106 to 3.5 × 107 spores·ml-1), sporulated over the larval cadavers, well grown at optimal temperature, and produced chitinolytic enzymes. Molecular analysis showed that isolates have nearly monophyletic characters and grouped under species of Beauveria bassiana. Different types of soil in Ethiopia are an important source of B. bassiana, and these isolates showed promising pathogenicity against T. absoluta, which is crucial for ecofriendly biopesticide development. Although isolates were nearly monophyletic in phylogenetic study, five of them were highly effective in the laboratory bioassays against T. absoluta; however, further field evaluation is required for mass production.
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DOI：
10.1016/j.bjm.2016.08.004
发表时间：
2017-07
期刊：
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
影响因子：
--
作者：
Dhawan M;Joshi N
通讯作者：
Joshi N
影响因子：
4.2
作者：
Garrido-Jurado I;Márquez M;Ortiz-Urquiza A;Santiago-Álvarez C;Iturriaga EA;Quesada-Moraga E;Monte E;Hermosa R
通讯作者：
Hermosa R
影响因子：
3
作者：
De Smedt C;Van Damme V;De Clercq P;Spanoghe P
通讯作者：
Spanoghe P
影响因子：
2.8
作者：
Holder, Diane J.;Kirkland, Brett H.;Keyhani, Nemat O.
通讯作者：
Keyhani, Nemat O.
影响因子：
1.1
作者：
Allegrucci, Natalia;Silvana Velazquez, Maria;Clara Scorsetti, Ana
通讯作者：
Clara Scorsetti, Ana