Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution.
Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution.
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DOI：
10.1186/1471-2180-11-84
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发表时间：
2011-04-26
期刊：
影响因子：
4.2
通讯作者：
Hermosa R
中科院分区：
文献类型：
--
作者：
Garrido-Jurado I;Márquez M;Ortiz-Urquiza A;Santiago-Álvarez C;Iturriaga EA;Quesada-Moraga E;Monte E;Hermosa R
球孢白僵菌（Beauveria bassiana）是一种具有昆虫病原性的变形真菌，目前被用作昆虫的生物防治剂。通过整合核大亚基核糖体基因（LSU rDNA） 3'端I族内含子插入模式和延伸因子1-α (EF1-α)系统发育信息，对57株球孢白弧菌（bbassiana）西班牙菌株进行了遗传结构和多样性分析。I组内含子基因型分析基于LSU的四个高度保守插入位点（Ec2653, Ec2449, Ec2066, Ec1921）。在16种可能的组合/基因型中，仅检测到4种，其中2种为优势组合/基因型，57株中分别含有44和9个成员。有趣的是，后两种基因型的成员在生长温度上表现出独特的差异。随后，EF1-α系统发育将大部分球孢白僵菌（bassiana s.s. (sensu stricto)）类群的菌株划分为5个分子亚群，在Ec1921位置均含有1个属于IC1亚型的I族内含子。还检查了一些参数，如热生长或起源（宿主、地理位置和气候条件），但一般没有发现关联。大多数西班牙球孢白僵菌分离株（77.2%）被归为一个主要的系统发育亚群，在世界范围内分布。然而，在气候变化小的近地理区域的西班牙分离株中也发现了高度的系统发育多样性。总的来说，西班牙球孢白僵菌分离株的分子分布与地理起源或气候特征之间没有相关性。
The entomopathogenic anamorphic fungus Beauveria bassiana is currently used as a biocontrol agent (BCA) of insects. Fifty-seven Beauveria bassiana isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ribosomal gene (LSU rDNA) and elongation factor 1-alpha (EF1-α) phylogenetic information, in order to assess the genetic structure and diversity of this Spanish collection of B. bassiana. Group I intron genotype analysis was based on the four highly conserved insertion sites of the LSU (Ec2653, Ec2449, Ec2066, Ec1921). Of the 16 possible combinations/genotypes, only four were detected, two of which were predominant, containing 44 and 9 members out of 57 isolates, respectively. Interestingly, the members of the latter two genotypes showed unique differences in their growth temperatures. In follow, EF1-α phylogeny served to classify most of the strains in the B. bassiana s.s. (sensu stricto) group and separate them into 5 molecular subgroups, all of which contained a group I intron belonging to the IC1 subtype at the Ec1921 position. A number of parameters such as thermal growth or origin (host, geographic location and climatic conditions) were also examined but in general no association could be found. Most Spanish B. bassiana isolates (77.2%) are grouped into a major phylogenetic subgroup with word-wide distribution. However, high phylogenetic diversity was also detected among Spanish isolates from close geographic zones with low climatic variation. In general, no correlation was observed between the molecular distribution and geographic origin or climatic characteristics where the Spanish B. bassiana isolates were sampled.
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影响因子：
3.1
作者：
Devi, K. Uma;Reineke, A.;Padmavathi, J.
通讯作者：
Padmavathi, J.
影响因子：
2.8
作者：
Bidochka, MJ;Menzies, FV;Kamp, AM
通讯作者：
Kamp, AM
影响因子：
2.8
作者：
Rehner, SA;Buckley, E
通讯作者：
Buckley, E
影响因子：
3.4
作者：
Berretta, MF;Lecuona, RE;Grau, O
通讯作者：
Grau, O
影响因子：
4.9
作者：
Meyling, Nicolai V.;Lubeck, Mette;Rehner, Stephen A.
通讯作者：
Rehner, Stephen A.