Gene therapy of murine teratocarcinoma: separate functions for insulin-like growth factors I and II in immunogenicity and differentiation.
Gene therapy of murine teratocarcinoma: separate functions for insulin-like growth factors I and II in immunogenicity and differentiation.
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鼠畸胎癌的基因治疗：胰岛素样生长因子 I 和 II 在免疫原性和分化中的不同功能。
DOI：
10.1073/pnas.91.13.6088
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发表时间：
1994
影响因子：
11.1
通讯作者：
Ilan,J
中科院分区：
文献类型：
--
作者：
Trojan,J;Johnson,TR;Rudin,SD;Blossey,BK;Kelley,KM;Shevelev,A;Abdul-Karim,FW;Anthony,DD;Tykocinski,ML;Ilan,J
畸胎癌是一种产生胚样肿瘤的种系癌，其结构源自三个胚胎层：中胚层、内胚层和外胚层。畸胎癌被广泛用作体外模型系统来研究哺乳动物胚胎发生过程中细胞决定和分化的调节。鼠胚胎癌 (EC) PCC3 细胞表达胰岛素样生长因子 I (IGF-I) 及其受体，而所有衍生肿瘤结构均表达 IGF-I 和 IGF-II 及其受体。因此，该系统有助于剖析这两种生长因子在 EC 分化过程中的作用。通过附加型反义策略，我们定义了 IGF-I 在致瘤性和逃避免疫监视中的作用。反义 IGF-I EC 转染子显示出可引发治疗性抗肿瘤免疫反应，并使远端部位的肿瘤消退。相比之下，IGF-II 显示可驱动 EC 细胞的决定和分化。由于IGF-I和IGF-II与I型受体结合并且用于IGF-II的反义序列不能与内源IGF-I转录物形成双链体，因此该受体不参与决定和分化。
Teratocarcinoma is a germ-line carcinoma giving rise to an embryoid tumor with structures derived from the three embryonic layers: mesoderm, endoderm, and ectoderm. Teratocarcinoma is widely used as an in vitro model system to study regulation of cell determination and differentiation during mammalian embryogenesis. Murine embryonic carcinoma (EC) PCC3 cells express insulin-like growth factor I(IGF-I) and its receptor, while all derivative tumor structures express IGF-I and IGF-II and their receptors. Therefore the system lends itself to dissect the role of these two growth factors during EC differentiation. With an episomal antisense strategy, we define a role for IGF-I in tumorigenicity and evasion of immune surveillance. Antisense IGF-I EC transfectants are shown to elicit a curative anti-tumor immune response with tumor regression at distal sites. In contrast, IGF-II is shown to drive determination and differentiation in EC cells. Since IGF-I and IGF-II bind to type I receptor and antisense sequence used for IGF-II cannot form duplex with endogenous IGF-I transcripts, it follows that this receptor is not involved in determination and differentiation.
人甲状腺腺瘤自分泌产生 IGF-1 的证据
影响因子：
4.1
作者：
D.Wynne Williams;E. Williams;D. Wynford‐Thomas
通讯作者：
D. Wynford‐Thomas
DOI：
--
发表时间：
1984
期刊：
影响因子：
--
作者：
鎌田 徹
通讯作者：
鎌田 徹