Dynamics of anterior-posterior axis formation in the developing mouse embryo.
Dynamics of anterior-posterior axis formation in the developing mouse embryo.
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DOI：
10.1038/ncomms1671
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发表时间：
2012-02-14
影响因子：
16.6
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中科院分区：
文献类型：
--
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前-后（AP）极性的发展是一个至关重要的过程，在小鼠中一直很难分析，因为它是在胚胎植入母体时发生的。为了克服这一障碍，我们建立了一个体外培养系统，使我们能够跟踪前内脏内胚层（AVE）的逐步发育，这对建立AP极性至关重要。在这里，我们使用这个系统来证明AVE起源于植入囊胚，但随后其他细胞获得了AVE特征。当卵筒从囊胚结构中出现时，这些“较老”和“较年轻”的AVE结构域合并。重要的是，我们发现AVE迁移是由表达最高水平AVE标记的细胞引导的，强调了AVE结构域内的不对称性决定了其迁移的方向。消融这些前导细胞可阻止AVE迁移，表明这些细胞对正确建立AP轴很重要。对小鼠胚胎中轴发育的详细分析还很有限。Morris等人开发了一种体外培养技术，能够实时观察前内脏内胚层的形成，并表明AVE亚域内细胞标记物的不对称性决定了AVE迁移的方向。
The development of an anterior–posterior (AP) polarity is a crucial process that in the mouse has been very difficult to analyse, because it takes place as the embryo implants within the mother. To overcome this obstacle, we have established an in-vitro culture system that allows us to follow the step-wise development of anterior visceral endoderm (AVE), critical for establishing AP polarity. Here we use this system to show that the AVE originates in the implanting blastocyst, but that additional cells subsequently acquire AVE characteristics. These 'older' and 'younger' AVE domains coalesce as the egg cylinder emerges from the blastocyst structure. Importantly, we show that AVE migration is led by cells expressing the highest levels of AVE marker, highlighting that asymmetry within the AVE domain dictates the direction of its migration. Ablation of such leading cells prevents AVE migration, suggesting that these cells are important for correct establishment of the AP axis. Detailed analysis of axis development in mouse embryo has been limited. Morris et al. developed an in vitro culture technique that enables the real-time observation of an anterior visceral endoderm formation and show that cell marker asymmetry within the AVE subdomain dictates the direction of the AVE migration.
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影响因子：
2.7
作者：
Torres-Padilla, Maria-Elena;Richardson, Lucy;Kolasinska, Paulina;Meilhac, Sigolene M.;Luetke-Eversloh, Merlin Verena;Zernicka-Goetz, Magdalena
通讯作者：
Zernicka-Goetz, Magdalena
影响因子：
2.6
作者：
Richardson, Lucy;Torres-Padilla, Maria-Elena;Zernicka-Goetz, Magdalena
通讯作者：
Zernicka-Goetz, Magdalena
影响因子：
64.8
作者：
Brennan, J;Lu, CC;Robertson, EJ
通讯作者：
Robertson, EJ
影响因子：
4.6
作者：
Mesnard, Daniel;Guzman-Ayala, Marcela;Constam, Daniel B.
通讯作者：
Constam, Daniel B.
影响因子：
2.7
作者：
HSU, YC
通讯作者：
HSU, YC