Aqueous cinnamon extract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential.
Aqueous cinnamon extract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential.
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DOI：
10.1186/1471-2407-10-210
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发表时间：
2010-05-18
期刊：
影响因子：
3.8
通讯作者：
Kaul-Ghanekar R
中科院分区：
文献类型：
--
作者：
Koppikar SJ;Choudhari AS;Suryavanshi SA;Kumari S;Chattopadhyay S;Kaul-Ghanekar R
化学预防，包括使用合成或天然制剂（单独或联合使用）来阻止人类癌症的发展，是一种极有前途的癌症预防策略。肉桂是应用最广泛的草药之一，具有多种生物活性，包括抗肿瘤活性。在本研究中，我们报道了肉桂对子宫颈癌细胞株SiHa的抗肿瘤活性。采用HPTLC法分析肉桂水提物（ACE-c）中肉桂醛的含量。采用Folin-Ciocalteau法测定ACE-c中多酚含量。采用MTT法进行细胞毒性分析。通过生长曲线、菌落形成和软琼脂实验，研究了肉桂对生长动力学的影响。采用RT-PCR和酶谱法分别对ACE-c处理后的细胞进行伤口愈合检测和基质金属蛋白酶-2 (MMP-2) mRNA和蛋白水平的表达。通过免疫印迹和共聚焦显微镜分析对照组和ACE-c处理样品中Her-2蛋白的表达。凋亡研究和钙信号分析通过流式细胞仪进行。通过JC-1染色研究肉桂处理细胞线粒体膜电位（Δψm）的损失，并通过共聚焦显微镜和FACS分析。肉桂以剂量依赖性的方式改变SiHa细胞的生长动力学。与对照细胞相比，ACE-c处理的细胞显示出较少的菌落数量。处理后的细胞表现出迁移潜力降低，这可能是由于MMP-2表达下调所致。有趣的是，Her-2癌蛋白的表达在ACE-c的存在下显著降低。肉桂提取物通过增加细胞内钙信号和线粒体膜电位的丧失诱导宫颈癌细胞凋亡。肉桂可作为一种有效的预防宫颈癌的化学药物。
Chemoprevention, which includes the use of synthetic or natural agents (alone or in combination) to block the development of cancer in human beings, is an extremely promising strategy for cancer prevention. Cinnamon is one of the most widely used herbal medicines with diverse biological activities including anti-tumor activity. In the present study, we have reported the anti-neoplastic activity of cinnamon in cervical cancer cell line, SiHa. The aqueous cinnamon extract (ACE-c) was analyzed for its cinnamaldehyde content by HPTLC analysis. The polyphenol content of ACE-c was measured by Folin-Ciocalteau method. Cytotoxicity analysis was performed by MTT assay. We studied the effect of cinnamon on growth kinetics by performing growth curve, colony formation and soft agar assays. The cells treated with ACE-c were analyzed for wound healing assay as well as for matrix metalloproteinase-2 (MMP-2) expression at mRNA and protein level by RT-PCR and zymography, respectively. Her-2 protein expression was analyzed in the control and ACE-c treated samples by immunoblotting as well as confocal microscopy. Apoptosis studies and calcium signaling assays were analyzed by FACS. Loss of mitochondrial membrane potential (Δψm) in cinnamon treated cells was studied by JC-1 staining and analyzed by confocal microscopy as well as FACS. Cinnamon alters the growth kinetics of SiHa cells in a dose-dependent manner. Cells treated with ACE-c exhibited reduced number of colonies compared to the control cells. The treated cells exhibited reduced migration potential that could be explained due to downregulation of MMP-2 expression. Interestingly, the expression of Her-2 oncoprotein was significantly reduced in the presence of ACE-c. Cinnamon extract induced apoptosis in the cervical cancer cells through increase in intracellular calcium signaling as well as loss of mitochondrial membrane potential. Cinnamon could be used as a potent chemopreventive drug in cervical cancer.
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影响因子：
5.8
作者：
Lee, SH;Lee, SY;Hong, JT
通讯作者：
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影响因子：
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作者：
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作者：
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Giovannini, Claudio