Space microgravity improves proliferation of human iPSC-derived cardiomyocytes.
Space microgravity improves proliferation of human iPSC-derived cardiomyocytes.
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太空微重力可改善人类 iPSC 来源的心肌细胞的增殖。
DOI：
10.1016/j.stemcr.2022.08.007
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发表时间：
2022-10-11
影响因子：
5.9
通讯作者：
Xu, Chunhui
中科院分区：
文献类型：
--
作者：
Rampoldi, Antonio;Forghani, Parvin;Li, Dong;Hwang, Hyun;Armand, Lawrence Christian;Fite, Jordan;Boland, Gene;Maxwell, Joshua;Maher, Kevin;Xu, Chunhui
在微重力下，细胞的性质发生了深刻的变化。然而，人类心脏祖细胞对空间微重力的反应尚不清楚。在这项研究中，我们评估了空间微重力对人类诱导多能干细胞（hiPSC）衍生的心脏祖细胞分化的影响，并与国际空间站（ISS）上的1G培养物进行了比较。将冷冻保存的3D心脏祖细胞在ISS上培养3周。与1G培养物相比，微重力培养物的球体大小大3倍，细胞核计数高20倍，增殖标记物的表达增加。在空间微重力条件下产生的高度富集的心肌细胞显示出改善的Ca 2+处理和增加的收缩相关基因的表达。心脏祖细胞短期暴露（3天）空间微重力上调基因参与细胞增殖，存活，心脏分化和收缩，在后期阶段与改善微重力培养相一致。这些结果表明，空间微重力增加了具有适当结构和功能的hiPSC-心肌细胞的增殖。微重力培养物中的跳动心肌细胞改善了Ca 2+处理微重力培养物上调了心脏收缩基因在这篇文章中，Xu和同事们表明，冷冻保存的3D心脏祖细胞在国际空间站（ISS）上分化为高度富集的心肌细胞。与ISS 1G细胞相比，ISS微重力细胞具有增加的增殖和改善的Ca 2+瞬变。短期微重力还增加了与增殖和心脏分化和收缩相关的基因的表达。
In microgravity, cells undergo profound changes in their properties. However, how human cardiac progenitors respond to space microgravity is unknown. In this study, we evaluated the effect of space microgravity on differentiation of human induced pluripotent stem cell (hiPSC)-derived cardiac progenitors compared with 1G cultures on the International Space Station (ISS). Cryopreserved 3D cardiac progenitors were cultured for 3 weeks on the ISS. Compared with 1G cultures, the microgravity cultures had 3-fold larger sphere sizes, 20-fold higher counts of nuclei, and increased expression of proliferation markers. Highly enriched cardiomyocytes generated in space microgravity showed improved Ca2+ handling and increased expression of contraction-associated genes. Short-term exposure (3 days) of cardiac progenitors to space microgravity upregulated genes involved in cell proliferation, survival, cardiac differentiation, and contraction, consistent with improved microgravity cultures at the late stage. These results indicate that space microgravity increased proliferation of hiPSC-cardiomyocytes, which had appropriate structure and function. Cryopreserved 3D hiPSC-cardiac progenitors differentiated efficiently in space Microgravity cultures had increased sphere sizes and cellular proliferation Beating cardiomyocytes in microgravity cultures had improved Ca2+ handling Microgravity cultures had upregulated genes in cardiac contraction In this article, Xu and colleagues show that cryopreserved 3D cardiac progenitors differentiated into highly enriched cardiomyocytes on the International Space Station (ISS). Compared with ISS 1G cells, ISS microgravity cells had increased proliferation and improved Ca2+ transients. Short-term microgravity also increased the expression of genes associated with proliferation and cardiac differentiation and contraction.
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