A systems genetics approach reveals environment-dependent associations between SNPs, protein coexpression, and drought-related traits in maize.
A systems genetics approach reveals environment-dependent associations between SNPs, protein coexpression, and drought-related traits in maize.
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DOI：
10.1101/gr.255224.119
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发表时间：
2020-11
期刊：
影响因子：
7
通讯作者：
Zivy M
中科院分区：
文献类型：
--
作者：
Blein-Nicolas M;Negro SS;Balliau T;Welcker C;Cabrera-Bosquet L;Nicolas SD;Charcosset A;Zivy M
在气候变化和人口增长的背景下，干旱对玉米产量的影响特别令人关切。然而，干旱响应机制的复杂性使得设计新的耐旱品种成为一项艰巨的任务，这将大大受益于更好地了解基因型-表型关系。为了提供这种关系的新见解，我们采用了系统遗传学方法，整合了高通量表型，蛋白质组学和基因组数据，从254个玉米杂交种在两种浇水条件下生长。使用关联遗传学和蛋白共表达分析，我们在两种条件下检测到超过22，000个pQTL，并确定了15个对蛋白质组具有潜在多效性效应的位点。我们表明，即使是轻微的缺水也会引起蛋白质组的深刻重塑，从而影响蛋白质共表达网络的结构，并对许多蛋白质丰度的遗传控制进行重新编程，包括那些参与应激反应的蛋白质。pQTL和生理生态性状QTL之间的共定位，发现主要在水分亏缺条件下，表明这种重编程也可能影响表型水平。最后，我们确定了几个候选基因，可能是负责胁迫反应蛋白的共表达和水分亏缺下的生理生态性状的变化。总之，我们的研究结果提供了新的见解耐旱性的分子机制，并提出了一些进一步的研究和育种途径。
The effect of drought on maize yield is of particular concern in the context of climate change and human population growth. However, the complexity of drought-response mechanisms makes the design of new drought-tolerant varieties a difficult task that would greatly benefit from a better understanding of the genotype–phenotype relationship. To provide novel insight into this relationship, we applied a systems genetics approach integrating high-throughput phenotypic, proteomic, and genomic data acquired from 254 maize hybrids grown under two watering conditions. Using association genetics and protein coexpression analysis, we detected more than 22,000 pQTLs across the two conditions and confidently identified 15 loci with potential pleiotropic effects on the proteome. We showed that even mild water deficit induced a profound remodeling of the proteome, which affected the structure of the protein coexpression network, and a reprogramming of the genetic control of the abundance of many proteins, including those involved in stress response. Colocalizations between pQTLs and QTLs for ecophysiological traits, found mostly in the water deficit condition, indicated that this reprogramming may also affect the phenotypic level. Finally, we identified several candidate genes that are potentially responsible for both the coexpression of stress response proteins and the variations of ecophysiological traits under water deficit. Taken together, our findings provide novel insights into the molecular mechanisms of drought tolerance and suggest some pathways for further research and breeding.
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影响因子：
7
作者：
Albertin, Warren;Marullo, Philippe;Sicard, Delphine
通讯作者：
Sicard, Delphine
DOI：
10.1126/science.1260793
发表时间：
2015-02-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Battle A;Khan Z;Wang SH;Mitrano A;Ford MJ;Pritchard JK;Gilad Y
通讯作者：
Gilad Y
影响因子：
3.4
作者：
Blein-Nicolas, Melisande;Xu, Hao;Zivy, Michel
通讯作者：
Zivy, Michel
影响因子：
4.6
作者：
Forestan C;Aiese Cigliano R;Farinati S;Lunardon A;Sanseverino W;Varotto S
通讯作者：
Varotto S
影响因子：
5.6
作者：
Golldack D;Li C;Mohan H;Probst N
通讯作者：
Probst N