Treslin, DUE-B, and GEMC1 cannot complement Sld3 mutants in fission yeast.
Treslin, DUE-B, and GEMC1 cannot complement Sld3 mutants in fission yeast.
复制标题
Treslin、DUE-B 和 GEMC1 不能补充裂殖酵母中的 Sld3 突变体。
DOI：
10.1111/j.1567-1364.2012.00794.x
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发表时间：
2012
影响因子：
3.2
通讯作者：
Xu,Yong-Jie
中科院分区：
文献类型：
--
作者：
Wang,Zhuo;Kim,Elaine;Leffak,Michael;Xu,Yong-Jie
真核生物DNA复制的起始是一个进化上保守的过程，包括两个不同的步骤：G1期复制起始处的预复制复合物的形成和S期预起始复合物（pre-ICs）的组装，后者导致复制解旋酶的激活。对于pre- ic在酵母中的组装，Sld2 - dpb11 - Sld3复合物的形成是一个关键事件，它需要细胞周期蛋白依赖性激酶磷酸化Sld2和Sld3。在哺乳动物中，RecQL4和TopBP1分别是Sld2和Dpb11极好的同源候选基因。在过去的一年里，三个topbp1相互作用的蛋白Treslin/Ticrr、GEMC1和du - b已经在后生动物中被鉴定为酵母Sld3的可能的功能同源物。为了验证这一假设，我们在裂变酵母中进行了几次互补试验。这些蛋白在温度敏感型ld3-10突变体和缺乏内源性Sld3的细胞中以不同水平表达。我们的结果表明，这些后生动物蛋白都不能挽救sl3突变体的生长缺陷。尽管这一结果可能有多种解释，但哺乳动物的解旋酶激活在进化过程中的复杂性可能与酵母不同，并且可能涉及与TopBP1相互作用的多个参与者。
Initiation of DNA replication in eukaryotes is an evolutionarily conserved process that involves two distinct steps: the formation of prereplication complexes at replication origins in G1 and the assembly of preinitiation complexes (pre-ICs) in S phase, which leads to activation of the replication helicase. For the assembly of pre-ICs in yeast, formation of the Sld2–Dpb11–Sld3 complex is a critical event that requires phosphorylation of Sld2 and Sld3 by cyclin-dependent kinase. In mammals, RecQL4 and TopBP1 are excellent ortholog candidates for Sld2 and Dpb11, respectively. In this past year, three TopBP1-interacting proteins Treslin/Ticrr, GEMC1, and DUE-B have been identified in metazoans as possible functional orthologs of the yeast Sld3. To test this hypothesis, we carried out several complementation tests in fission yeast. The proteins were expressed at various levels in the temperature-sensitivesld3-10mutant and in cells that lack endogenous Sld3. Our result showed that none of these metazoan proteins could rescue growth defect of thesld3mutants. Although the result may have several interpretations, it is possible that the helicase activation in mammals has diverged in complexity during evolution from that in yeasts and may involve multiple players that interact with TopBP1.
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影响因子：
64.8
作者：
Tanaka, Seiji;Umemori, Toshiko;Araki, Hiroyuki
通讯作者：
Araki, Hiroyuki
影响因子：
11.4
作者：
Kamimura, Y;Tak, YS;Araki, H
通讯作者：
Araki, H
通过与CDC45和杜松子蛋白缔合对MCM2-7解旋酶的激活。
影响因子：
16
作者：
Ilves I;Petojevic T;Pesavento JJ;Botchan MR
通讯作者：
Botchan MR
影响因子：
8
作者：
Yamane, K;Tsuruo, T
通讯作者：
Tsuruo, T
影响因子：
10.5
作者：
Sansam, Christopher L.;Cruz, Nelly M.;Lees, Jacqueline A.
通讯作者：
Lees, Jacqueline A.