Vascular endothelial growth factor and fibroblast growth factor 2 delivery from spinal cord bridges to enhance angiogenesis following injury.
Vascular endothelial growth factor and fibroblast growth factor 2 delivery from spinal cord bridges to enhance angiogenesis following injury.
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DOI：
10.1002/jbm.a.33112
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发表时间：
2011-09-01
影响因子：
4.9
通讯作者：
Shea, Lonnie D.
中科院分区：
文献类型：
--
作者：
De Laporte, Laura;des Rieux, Anne;Tuinstra, Hannah M.;Zelivyanskaya, Marina L.;De Clerck, Nora M.;Postnov, Andrei A.;Preat, Veronique;Shea, Lonnie D.
宿主对脊髓损伤的反应可导致可诱导细胞死亡的缺血性环境，并限制促进脊髓再生的细胞移植方法。研究了提供VEGF和FGF-2的局部和持续释放的脊髓桥促进损伤内血管生成和神经生长的能力。使用气体发泡/颗粒浸出技术通过聚（丙交酯-共-乙交酯）微球的融合制造桥，并且通过封装到微球中和/或在发泡之前与微球混合来将蛋白质并入。与混合方法相比，包封减少了浸出过程中的损失，并具有较慢的蛋白质释放，而VEGF比FGF-2更快地释放。体内植入负载VEGF的桥在1周时增强了损伤内VEGF的水平，植入后6周，释放VEGF和FGF-2的桥增加了内皮细胞的浸润和血管的形成。此外，在桥内观察到大量神经丝染色;然而，在有或没有蛋白质的桥之间没有观察到显著差异。释放血管生成因子的桥可以提供一种克服限制再生的缺血环境的方法和基于细胞移植的方法。
The host response to spinal cord injury can lead to an ischemic environment that can induce cell death and limits cell transplantation approaches to promote spinal cord regeneration. Spinal cord bridges that provide a localized and sustained release of VEGF and FGF-2 were investigated for their ability to promote angiogenesis and nerve growth within the injury. Bridges were fabricated by fusion of poly(lactide-co-glycolide) microspheres using a gas foaming/particulate leaching technique, and proteins were incorporated by encapsulation into the microspheres and/or mixing with the microspheres before foaming. Compared to the mixing method, encapsulation reduced the losses during leaching and had a slower protein release, while VEGF was released more rapidly than FGF-2. In vivo implantation of bridges loaded with VEGF enhanced the levels of VEGF within the injury at one week, and bridges releasing VEGF and FGF-2 increased the infiltration of endothelial cells and the formation of blood vessel at 6 weeks post implantation. Additionally, substantial neurofilament staining was observed within the bridge; however, no significant difference was observed between bridges with or without protein. Bridges releasing angiogenic factors may provide an approach to overcome an ischemic environment that limits regeneration and cell transplantation based approaches.
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