Identification of the RNA polymerase I-RNA interactome.
Identification of the RNA polymerase I-RNA interactome.
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DOI：
10.1093/nar/gky779
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发表时间：
2018-11-16
影响因子：
14.9
通讯作者：
Willis AE
中科院分区：
文献类型：
--
作者：
Piñeiro D;Stoneley M;Ramakrishna M;Alexandrova J;Dezi V;Juke-Jones R;Lilley KS;Cain K;Willis AE
核糖体的生物合成是一个由许多核糖体组装因子协调的复杂过程。尽管已知参与该过程的许多蛋白质具有RNA结合活性，但与前体核糖体RNA相互作用的蛋白质的全部谱目前尚不清楚。为了更好地了解RNA-蛋白质相互作用在多大程度上有可能控制核糖体生物合成，我们使用RNA亲和分离结合蛋白质组学来测量当rRNA转录被阻断时发生的RNA-蛋白质相互作用的变化。我们的分析确定了457个核RNA结合蛋白中的211个在RNA聚合酶I（RNAPI）抑制后RNA-蛋白质相互作用减少>3倍。我们将这211种RNA结合蛋白命名为RNAPI RNA相互作用体。正如预期的那样，RNAPI RNA相互作用组高度富集核仁蛋白和与核糖体生物发生相关的蛋白。从相互作用组中选择的蛋白质被证明是核仁的位置，并具有依赖于RNAPI活性的RNA结合活性。此外，我们的数据表明，两种蛋白质，这是所需的rRNA成熟，AATF和NGDN，并形成RNA相互作用组的一部分，都缺乏典型的RNA结合结构域，但仍是新的前rRNA结合蛋白。
Ribosome biogenesis is a complex process orchestrated by a host of ribosome assembly factors. Although it is known that many of the proteins involved in this process have RNA binding activity, the full repertoire of proteins that interact with the precursor ribosomal RNA is currently unknown. To gain a greater understanding of the extent to which RNA-protein interactions have the potential to control ribosome biogenesis, we used RNA affinity isolation coupled with proteomics to measure the changes in RNA-protein interactions that occur when rRNA transcription is blocked. Our analysis identified 211 out of 457 nuclear RNA binding proteins with a >3-fold decrease in RNA-protein interaction after inhibition of RNA polymerase I (RNAPI). We have designated these 211 RNA binding proteins as the RNAPI RNA interactome. As expected, the RNAPI RNA interactome is highly enriched for nucleolar proteins and proteins associated with ribosome biogenesis. Selected proteins from the interactome were shown to be nucleolar in location and to have RNA binding activity that was dependent on RNAPI activity. Furthermore, our data show that two proteins, which are required for rRNA maturation, AATF and NGDN, and which form part of the RNA interactome, both lack canonical RNA binding domains and yet are novel pre-rRNA binding proteins.
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影响因子：
12.4
作者：
通讯作者：
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影响因子：
14.9
作者：
Bammert L;Jonas S;Ungricht R;Kutay U
通讯作者：
Kutay U
影响因子：
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作者：
Beckmann BM;Horos R;Fischer B;Castello A;Eichelbaum K;Alleaume AM;Schwarzl T;Curk T;Foehr S;Huber W;Krijgsveld J;Hentze MW
通讯作者：
Hentze MW
影响因子：
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作者：
Castello, Alfredo;Horos, Rastislav;Hentze, Matthias W.
通讯作者：
Hentze, Matthias W.
影响因子：
16
作者：
Mayer, Christine;Schmitz, Kerstin-Maike;Santoro, Raffaella
通讯作者：
Santoro, Raffaella