Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy.
Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy.
复制标题
基于 NMR 的组织生物标志物定量代谢组学及其在体内磁共振波谱分析中的转化。
DOI：
10.1007/978-1-4939-9236-2_23
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Agarwal,Rajesh
中科院分区：
文献类型：
--
作者：
Serkova,NatalieJ;Davis,DeniseM;Steiner,Jenna;Agarwal,Rajesh
Nuclear magnetic resonance (NMR) spectroscopy is an established analytical platform for analyzing metabolic profiles of cells, tissues, and body fluids. There are several advantages in introducing an NMR-based study design into metabolomics studies, including a fast and comprehensive detection, characterization, and quantification of dozens of endogenous metabolites in a single NMR spectrum. Quantitative proton1H-NMR is the most useful NMR-based platform for metabolomics. The frozen tissues can be analyzed noninvasively using a high-resolution magic angle spinning (HR-MAS)1H-NMR spectroscopy; or several extraction techniques can be applied to detect additional metabolites using a conventional liquid-based NMR technique. In this chapter, we report on tissue collection, handling, extraction methods, and1H-NMR acquisition protocols developed in the past decades for a precise and quantitative NMR-metabolomics approach. The NMR acquisition protocols (both HR-MAS and conventional1H-NMR spectroscopy) and spectral analysis steps are also presented. Since NMR can be applied “in vivo” using horizontal bore MRI scanners, several in vivo sequences for localized1H-MRS (magnetic resonance spectroscopy) are presented which can be directly applied for noninvasive detection of brain metabolites.
影响因子：
4.1
作者：
Tzika, AA;Cheng, LL;Black, PM
通讯作者：
Black, PM
影响因子：
4.6
作者：
Rudolph, Michael C.;McManaman, James L.;Neville, Margaret C.
通讯作者：
Neville, Margaret C.
影响因子：
3.8
作者：
Niemann, Claus U.;Choi, Soojinna;Maher, Jacquelyn J.
通讯作者：
Maher, Jacquelyn J.