A microdevice for studying intercellular electromechanical transduction in adult cardiac myocytes.
A microdevice for studying intercellular electromechanical transduction in adult cardiac myocytes.
复制标题
用于研究成人心肌细胞细胞间机电转导的微型装置。
DOI：
10.1039/c3lc50414j
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发表时间：
2013
期刊：
影响因子：
6.1
通讯作者：
Zhao,Yi
中科院分区：
文献类型：
--
作者：
Zhang,Xu;Wang,Qian;Gablaski,Brian;Zhang,Xiaojin;Lucchesi,Pamela;Zhao,Yi
Intercellular electromechanical transduction in adult cardiac myocytes plays an important role in regulating heart function. The efficiency of intercellular electromechanical transduction has so far been investigated only to a limited extent, which is largely due to the lack of appropriate tools that can quantitatively assess the contractile performance of interconnected adult cardiac myocytes. In this paper we report a microengineered device that is capable of applying electrical stimulation to the selected adult cardiac myocyte in a longitudinally connected cell doublet and quantifying the intercellular electromechanical transduction by measuring the contractile performance of stimulated and un-stimulated cells in the same doublet. The capability of applying selective electrical stimulation on only one cell in the doublet is validated by examining cell contractile performance while blocking the intercellular communication. Quantitative assessment of cell contractile performance in isolated adult cardiac myocytes is performed by measuring the change in cell length. The proof-of-concept assessment of gap junction performance shows that the device is useful in studying the efficiency of gap junctions in adult cardiac myocytes, which is most relevant to the synchronized pumping performance of native myocardium. Collectively, this work provides a quantitative tool for studying intercellular electromechanical transduction and is expected to develop a comprehensive understanding of the role of intercellular communication in various heart diseases.
影响因子：
56.9
作者：
GOOD, MF;MILLER, LH;CARTER, R
通讯作者：
CARTER, R
影响因子：
2.4
作者：
HAMILTON, AJ;DAVIES, CS;SINDEN, RE
通讯作者：
SINDEN, RE
史氏按蚊中肠孢子发生期间疟原虫恶性疟原虫的环子孢子蛋白的免疫金定位。
影响因子：
6.6
作者：
G. Posthuma;Jacques F. Meis;J. Verhave;M. Hollingdale;T. Ponnudurai;J. Meuwissen;H. Geuze
通讯作者：
H. Geuze