Protists modulate active bacterial community composition in paddy field soils
Protists modulate active bacterial community composition in paddy field soils
复制标题
原生生物调节稻田土壤中的活性细菌群落组成
DOI：
10.1007/s00374-023-01733-5
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发表时间：
2023
影响因子：
6.5
通讯作者：
Asiloglu R
中科院分区：
文献类型：
--
作者：
Fujino;M.;Suzuki;K.;Harada;N;Asiloglu R
包括稻田在内的土壤生态系统中的细菌群落受到捕食性原生动物自上而下的强烈控制。然而，目前关于细菌群落自上而下调控的知识仅来自基于DNA的分子方法，这些方法在区分活细胞和死细胞方面存在局限性。在这里，我们比较了基于DNA和RNA的高通量测序，以估计捕食性原生生物对细菌群落组成的影响。研究了3个原生生物菌株对两种不同理化性质稻田土壤中活性（RNA）和总（DNA）细菌群落组成的影响。结果表明：（1）原生生物对细菌群落的控制具有种特异性;（2）土壤类型是决定微生物营养相互作用结果的重要因素;（3）基于DNA的方法可能高估原生生物对厚壁菌门的影响，而低估原生生物对变形菌门和拟杆菌门的影响。尽管过度和低估的特定细菌类群，DNA和RNA为基础的结果提供了类似的模式原生生物的捕食作用，这使得DNA和RNA为基础的方法强大的工具，以评估营养相互作用。然而，在基于DNA的研究中，稻田细菌群落的自上而下的调控应仔细评估，特别是对于属于变形菌门和厚壁菌门的细菌类群。
Bacterial communities in the soil ecosystem including paddy fields are strongly controlled by the top-down force of predatory protists. However, the current knowledge on top-down regulation of bacterial communities exclusively comes from DNA-based molecular methods that have limitations to differentiate between alive and dead cells. Here, we compared DNA- and RNA-based high-throughput sequencing to estimate the effect of predatory protists on bacterial community composition. We studied the effect of three protist isolates on active (RNA-based) and total (DNA-based) bacterial community composition in two paddy field soils with different physicochemical properties. The main results are (1) protists control bacterial communities with a species-specific effect, (2) soil type is an important factor determining the outcome of the microbial trophic interactions, and (3) DNA-based methods are likely to overestimate the effect of protists for Firmicutes, while it may cause an underestimation of the protist-effect on Proteobacteria and Bacteroidota. Despite the over- and underestimation of specific bacterial taxa, both DNA- and RNA-based results provided similar patterns for the predatory effect of protists, which makes both DNA- and RNA-based methods powerful tools to evaluate trophic interactions. Nevertheless, the top-down regulation of paddy field bacterial communities in DNA-based studies should be carefully evaluated, especially for the bacterial taxa belonging to Proteobacteria and Firmicutes.
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影响因子：
5.2
作者：
Gong J;Qing Y;Zou S;Fu R;Su L;Zhang X;Zhang Q
通讯作者：
Zhang Q
淡水和土壤裸阿米巴的新钥匙及培养说明
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
F. Pagé
通讯作者：
F. Pagé
更正：原生生物：湿地稻田土壤中隐藏的生态系统参与者
影响因子：
6.5
作者：
J. Murase;Rasit Asiloglu
通讯作者：
Rasit Asiloglu
影响因子：
5.1
作者：
Flues, Sebastian;Bass, David;Bonkowski, Michael
通讯作者：
Bonkowski, Michael
根际细菌上原生动物的放牧改变了拟南芥的生长和繁殖
影响因子：
9.7
作者：
K. Krome;K. Rosenberg;M. Bonkowski;S. Scheu
通讯作者：
S. Scheu