Mutation of PEB4 alters the outer membrane protein profile of Campylobacter jejuni.
Mutation of PEB4 alters the outer membrane protein profile of Campylobacter jejuni.
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DOI：
10.1111/j.1574-6968.2009.01795.x
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发表时间：
2009-11
影响因子：
2.1
通讯作者：
Thompson SA
中科院分区：
文献类型：
--
作者：
Rathbun KM;Thompson SA
空肠弯曲菌是全球范围内引发人类肠胃炎的一个重要病因。为了进一步明确影响其感染性的细菌因素，研究发现Cj0596在空肠弯曲菌的毒力方面发挥着作用。Cj0596是一种周质伴侣蛋白，与其他细菌中参与外膜蛋白（OMP）折叠的蛋白质类似。与野生型细菌相比，cj0596基因的突变导致8种外膜蛋白水平发生改变。用野生型cj0596基因替换突变的cj0596基因后，外膜蛋白图谱恢复到野生型状态。cj0596突变体中改变的外膜蛋白图谱伴随着几种毒力特性的显著变化，包括自凝集能力增强、对多种抗菌剂的敏感性增加以及生物被膜形成增多。总之，cj0596基因的突变改变了空肠弯曲菌的外膜蛋白图谱，并导致与空肠弯曲菌致病机制相关的外膜蛋白表型发生变化。
Campylobacter jejuni is a significant cause of human gastroenteritis worldwide. In an attempt to further define bacterial factors that influence infectivity, Cj0596 was identified as playing a role in C. jejuni virulence. Cj0596 is a periplasmic chaperone that is similar to proteins involved in outer membrane protein (OMP) folding in other bacteria. Mutation of cj0596 caused an alteration in the levels of eight OMPs, compared to wild-type bacteria. Replacement of the cj0596 mutation with the wild-type cj0596 gene restored a wild-type OMP profile. The altered OMP profile in the cj0596 mutant was accompanied by significant changes in several virulence properties, including an increase in the ability to autoagglutinate, increased susceptibility to several antimicrobial agents, and increased biofilm formation. In summary, mutation of cj0596 alters the C. jejuni OMP profile and leads to changes in OMP-related phenotypes involved in C. jejuni pathogenesis.
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DOI：
10.1099/mic.0.024539-0
发表时间：
2009-03
期刊：
Microbiology (Reading, England)
影响因子：
--
作者：
Hobb RI;Fields JA;Burns CM;Thompson SA
通讯作者：
Thompson SA
影响因子：
3.2
作者：
Kalmokoff, Martin;Lanthier, Patricia;Szymanski, Christine M.
通讯作者：
Szymanski, Christine M.
影响因子：
4.9
作者：
Lin, J;Michel, LO;Zhang, QJ
通讯作者：
Zhang, QJ
影响因子：
3.2
作者：
Candon, Heather L.;Allan, Brenda J.;Gaynor, Erin C.
通讯作者：
Gaynor, Erin C.
影响因子：
3.6
作者：
Knutton, S;Shaw, RK;Zorgani, AA
通讯作者：
Zorgani, AA