L-arginine mediated renaturation enhances yield of human, α6 Type IV collagen non-collagenous domain from bacterial inclusion bodies.
L-arginine mediated renaturation enhances yield of human, α6 Type IV collagen non-collagenous domain from bacterial inclusion bodies.
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DOI：
10.2174/092986612802762750
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发表时间：
2012-10
影响因子：
1.6
通讯作者：
Sudhakar YA
中科院分区：
文献类型：
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作者：
Gunda V;Boosani CS;Verma RK;Guda C;Sudhakar YA
抗血管生成的羧基末端非胶原结构域（NC 1）来源于人IV型胶原α 6链，[α6（IV）NC 1]或hexastatin，较早时在细菌系统中使用不同的重组表达方法获得。然而，L-精氨酸介导的复性在提高该蛋白质从细菌包涵体的相对产率方面的作用尚未被评估。本研究采用直接搅拌和使用L-精氨酸和不同尺寸排阻层析基质的柱上复性方法来提高从细菌包涵体中纯化重组α6（IV）NC 1的溶解度。该方法能够从包涵体中纯化更高量的可溶性蛋白，其抑制内皮细胞增殖、迁移和管形成。因此，L-精氨酸介导的复性在从细菌包涵体获得更高产量的可溶性生物活性NC 1结构域的范围进行了评估。
The anti-angiogenic, carboxy terminal non-collagenous domain (NC1) derived from human Collagen type IV alpha 6 chain, [α6(IV)NC1] or hexastatin, was earlier obtained using different recombinant methods of expression in bacterial systems. However, the effect of L-arginine mediated renaturation in enhancing the relative yields of this protein from bacterial inclusion bodies has not been evaluated. In the present study, direct stirring and on-column renaturation methods using L-arginine and different size exclusion chromatography matrices were applied for enhancing the solubility in purifying the recombinant α6(IV)NC1 from bacterial inclusion bodies. This methodology enabled purification of higher quantities of soluble protein from inclusion bodies, which inhibited endothelial cell proliferation, migration and tube formation. Thus, the scope for L-arginine mediated renaturation in obtaining higher yields of soluble, biologically active NC1 domain from bacterial inclusion bodies was evaluated.
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影响因子：
6.4
作者：
Mundel, Thomas M.;Yliniemi, Anna-Maija;Kalluri, Raghu
通讯作者：
Kalluri, Raghu
影响因子：
8
作者：
O'Callaghan, CA;Tormo, J;Jones, EY
通讯作者：
Jones, EY
影响因子：
4.8
作者：
Petitclerc, E;Boutaud, A;Brooks, PC
通讯作者：
Brooks, PC
影响因子：
8
作者：
Reddy, RC;Lilie, H;Lange, C
通讯作者：
Lange, C
DOI：
10.1101/sqb.2002.67.255
发表时间：
2002-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
Kalluri, R
通讯作者：
Kalluri, R