Aggregation of Vibrio cholerae by Cationic Polymers Enhances Quorum Sensing but Overrides Biofilm Dissipation in Response to Autoinduction.
Aggregation of Vibrio cholerae by Cationic Polymers Enhances Quorum Sensing but Overrides Biofilm Dissipation in Response to Autoinduction.
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DOI：
10.1021/acschembio.8b00815
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发表时间：
2018-10-19
影响因子：
4
通讯作者：
Krachler AM
中科院分区：
文献类型：
--
作者：
Perez-Soto N;Creese O;Fernandez-Trillo F;Krachler AM
霍乱弧菌是一种在水生环境中发现的革兰氏阴性细菌，是一种全球性的人类病原体。它在宿主相关和环境生活方式之间的转变涉及对生态位特异性表型的严格调节，如运动性、生物膜形成和毒力。霍乱弧菌从宿主向环境扩散的转变通常涉及毒力的抑制和生物膜群落的扩散。与这种自然发生的转变相反，阳离子聚合物引起的细菌聚集引发了独特的反应，即抑制毒力基因表达，同时还引发霍乱弧菌形成生物膜，这是一种人工特征组合，可能非常有助于结合和中和污染水中的病原体。在这里，我们着手揭示这种聚合物触发的细菌行为的机制基础。我们发现，细菌聚合物聚集体进行快速的自诱导，并实现群体感应在细菌密度远低于所需的自诱导在没有聚合物。我们证明，这种诱导的群体感应是由于快速形成的自诱导物梯度和局部增强的自诱导物浓度内的细菌集群，以及刺激CAI-1和AI-2生产的聚集细菌。我们进一步发现，聚合物引起生物膜特异性调节剂VpsR和生物膜结构蛋白RbmA的诱导，绕过了自诱导过程中通常对生物膜的抑制。总的来说，这项研究强调了合成材料可用于交叉天然细菌反应，以实现具有潜在有用应用的表型组合。
Vibrio cholerae is a Gram-negative bacterium found in aquatic environments and a human pathogen of global significance. Its transition between host-associated and environmental lifestyles involves the tight regulation of niche-specific phenotypes such as motility, biofilm formation, and virulence. V. cholerae’s transition from the host to environmental dispersal usually involves suppression of virulence and dispersion of biofilm communities. In contrast to this naturally occurring transition, bacterial aggregation by cationic polymers triggers a unique response, which is to suppress virulence gene expression while also triggering biofilm formation by V. cholerae, an artificial combination of traits that is potentially very useful to bind and neutralize the pathogen from contaminated water. Here, we set out to uncover the mechanistic basis of this polymer-triggered bacterial behavior. We found that bacteria–polymer aggregates undergo rapid autoinduction and achieve quorum sensing at bacterial densities far below those required for autoinduction in the absence of polymers. We demonstrate this induction of quorum sensing is due both to a rapid formation of autoinducer gradients and local enhancement of autoinducer concentrations within bacterial clusters as well as the stimulation of CAI-1 and AI-2 production by aggregated bacteria. We further found that polymers cause an induction of the biofilm-specific regulator VpsR and the biofilm structural protein RbmA, bypassing the usual suppression of biofilm during autoinduction. Overall, this study highlights that synthetic materials can be used to cross-wire natural bacterial responses to achieve a combination of phenotypes with potentially useful applications.
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影响因子：
3.2
作者：
Shikuma, Nicholas J.;Fong, Jiunn C. N.;Yildiz, Fitnat H.
通讯作者：
Yildiz, Fitnat H.
DOI：
10.1038/nbt1183-784
发表时间：
1983-01-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
SIMON, R;PRIEFER, U;PUHLER, A
通讯作者：
PUHLER, A
影响因子：
3.2
作者：
DUNLAP, PV;GREENBERG, EP
通讯作者：
GREENBERG, EP
影响因子：
3.6
作者：
BASSLER, BL;WRIGHT, M;SILVERMAN, MR
通讯作者：
SILVERMAN, MR
DOI：
10.1073/pnas.81.13.4154
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ENGEBRECHT, J;SILVERMAN, M
通讯作者：
SILVERMAN, M