C. elegans midbodies are released, phagocytosed and undergo LC3-dependent degradation independent of macroautophagy.
C. elegans midbodies are released, phagocytosed and undergo LC3-dependent degradation independent of macroautophagy.
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DOI：
10.1242/jcs.190223
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发表时间：
2016-10-15
影响因子：
4
通讯作者：
Wehman AM
中科院分区：
文献类型：
--
作者：
Fazeli G;Trinkwalder M;Irmisch L;Wehman AM
在动物中，当子细胞通过分裂而分离时，中体协调胞质分裂的结束。中间体被认为是由巨自噬隔离，但最近的证据表明，中间体主要是释放和吞噬。然而，尚不清楚自噬蛋白是否在中体吞噬体降解中发挥作用。使用蛋白质降解试验，我们表明，中间体释放秀丽隐杆线虫。已知释放的中间体通过肌动蛋白驱动的吞噬作用被内化，我们表明这需要RAB-5 GTdR将III类磷酸肌醇3-激酶（PI 3 K）复合物定位在皮质。自噬相关蛋白，包括Beclin 1同源物BEC-1和Atg 8/LC 3家族成员LGG-1和LGG-2，定位于中体吞噬体周围，并且是中体降解所需的。相比之下，巨自噬特异性所需的蛋白质，如Ep-51和EPG-8（分别与ULK 1/Atg 1和Atg 14同源），不是中间体降解所需的。这些数据表明，C. elegans的中间体被LC 3相关的吞噬作用（macroautophagy）降解，而不是大自噬。我们的研究结果调和了吞噬和自噬蛋白作用的两种流行模型，建立了自噬蛋白在中间体降解中的新的非经典作用。摘要：自噬蛋白是中间体降解所必需的。In C.在线虫中，Atg 8/LC 3家族蛋白在吞噬体成熟期间而不是在大自噬期间起作用。
In animals, the midbody coordinates the end of cytokinesis when daughter cells separate through abscission. The midbody was thought to be sequestered by macroautophagy, but recent evidence suggests that midbodies are primarily released and phagocytosed. It was unknown, however, whether autophagy proteins play a role in midbody phagosome degradation. Using a protein degradation assay, we show that midbodies are released in Caenorhabditis elegans. Released midbodies are known to be internalized by actin-driven phagocytosis, which we show requires the RAB-5 GTPase to localize the class III phosphoinositide 3-kinase (PI3K) complex at the cortex. Autophagy-associated proteins, including the Beclin 1 homolog BEC-1 and the Atg8/LC3-family members LGG-1 and LGG-2, localize around the midbody phagosome and are required for midbody degradation. In contrast, proteins required specifically for macroautophagy, such as UNC-51 and EPG-8 (homologous to ULK1/Atg1 and Atg14, respectively) are not required for midbody degradation. These data suggest that the C. elegans midbody is degraded by LC3-associated phagocytosis (LAP), not macroautophagy. Our findings reconcile the two prevailing models on the role of phagocytic and autophagy proteins, establishing a new non-canonical role for autophagy proteins in midbody degradation. Summary: Autophagy proteins are required for the degradation of midbodies. In C. elegans, Atg8/LC3-family proteins act during phagosome maturation rather than during macroautophagy.
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影响因子：
19
作者：
Funderburk SF;Wang QJ;Yue Z
通讯作者：
Yue Z
DOI：
10.1083/jcb.201306036
发表时间：
2013-11-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Green RA;Mayers JR;Wang S;Lewellyn L;Desai A;Audhya A;Oegema K
通讯作者：
Oegema K
影响因子：
64.5
作者：
Kim JY;Zhao H;Martinez J;Doggett TA;Kolesnikov AV;Tang PH;Ablonczy Z;Chan CC;Zhou Z;Green DR;Ferguson TA
通讯作者：
Ferguson TA
影响因子：
4
作者：
Crowell, Elizabeth Faris;Gaffuri, Anne-Lise;Echard, Arnaud
通讯作者：
Echard, Arnaud
影响因子：
13.3
作者：
Isakson, Pauline;Lystad, Alf Hakon;Simonsen, Anne
通讯作者：
Simonsen, Anne