The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules.
The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules.
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DOI：
10.1083/jcb.201306036
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发表时间：
2013-11-11
期刊：
影响因子：
--
通讯作者：
Oegema K
中科院分区：
文献类型：
--
作者：
Green RA;Mayers JR;Wang S;Lewellyn L;Desai A;Audhya A;Oegema K
中隔，但不是中间体微管，是重要的子细胞胞质分离和ESCRT依赖的中间体环释放过程中的分裂。脱落完成胞质分裂形成两个子细胞。虽然可以通过基于微管的中间体从内到外或通过收缩环衍生的中间体环从外到内组织解离，但假设中间体微管支架化解离机器。在本文中，我们评估的贡献，中间体微管与中间体环的秀丽隐杆线虫胚胎。我们表明，发生在两个阶段的剥离。首先，子细胞中的细胞质变得孤立，与细胞间桥的形成相一致;通过这个阶段的适当进展需要隔膜（一种中间体环组分），而不是运输（ESCRT）机制所需的膜重塑内体分选复合物。其次，在随后的细胞分裂过程中，中间体和中间体环被释放到特定的子细胞中;这个阶段需要septins和ESCRT机制。令人惊讶的是，在这两个阶段，中间体微管都被破坏了。这些结果描绘了不同的步骤，并强调了中央的作用，而不是中间体微管，在他们的执行过程中的中间体环。
The septins, but not midbody microtubules, are important for daughter cell cytoplasmic isolation and ESCRT-dependent midbody ring release during abscission. Abscission completes cytokinesis to form the two daughter cells. Although abscission could be organized from the inside out by the microtubule-based midbody or from the outside in by the contractile ring–derived midbody ring, it is assumed that midbody microtubules scaffold the abscission machinery. In this paper, we assess the contribution of midbody microtubules versus the midbody ring in the Caenorhabditis elegans embryo. We show that abscission occurs in two stages. First, the cytoplasm in the daughter cells becomes isolated, coincident with formation of the intercellular bridge; proper progression through this stage required the septins (a midbody ring component) but not the membrane-remodeling endosomal sorting complex required for transport (ESCRT) machinery. Second, the midbody and midbody ring are released into a specific daughter cell during the subsequent cell division; this stage required the septins and the ESCRT machinery. Surprisingly, midbody microtubules were dispensable for both stages. These results delineate distinct steps during abscission and highlight the central role of the midbody ring, rather than midbody microtubules, in their execution.
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