MiRNA expression profiles in the brains of mice infected with scrapie agents 139A, ME7 and S15.
MiRNA expression profiles in the brains of mice infected with scrapie agents 139A, ME7 and S15.
复制标题
感染瘙痒病病原体 139A、ME7 和 S15 的小鼠大脑中的 miRNA 表达谱
DOI：
10.1038/emi.2016.120
复制
发表时间：
2016-11-09
影响因子：
13.2
通讯作者：
Dong, Xiao-Ping
中科院分区：
文献类型：
--
作者：
Gao, Chen;Wei, Jing;Zhang, Bao-Yun;Shi, Qiang;Chen, Cao;Wang, Jing;Shi, Qi;Dong, Xiao-Ping
MicroRNA（miRNA）是一类非编码的内源性小分子单链RNA，通过降解或翻译mRNA靶点来调节互补mRNA。通常，miRNAs显示出显著的细胞和组织特异性。最近，已经报道了患有某些神经退行性疾病（包括朊病毒疾病）的患者的大脑中的一组miRNA的改变。在这项研究中，使用深度测序技术，比较分析了在终末期感染羊瘙痒病病原体139 A，ME 7和S15的小鼠脑中的miRNA表达谱。总的来说，与年龄匹配的正常对照组相比，在139 A-，ME 7-和S15-感染小鼠的合并脑样品中分别鉴定出57，94和135个差异表达的miRNA。其中22个在所有三种感染模型的脑中普遍增加，14个普遍减少。此外，还普遍观察到两种新的miRNA表达的减少。对三种感染小鼠模型大脑中六种随机选择的常见增加和减少的miRNAs以及两种新型miRNAs进行定量PCR和逆转录分析，证实表达模式与深度测序数据相当。差异表达的miRNAs的KEGG分析揭示了在所有三种类型的感染动物中涉及相似的途径。对这些miRNA谱的综合分析不仅为理解朊病毒生物学提供了有用的线索，而且有助于寻找朊病毒疾病的可能诊断标志物。Emerging Microbes & Infections（2016）5，e115; doi：10.1038/emi.2016.120;在线发表2016年11月9日
MicroRNA (miRNA) is a class of non-coding endogenous small-molecule single-stranded RNA that regulates complementary mRNA through degradation or translation of the mRNA targets. Usually, miRNAs show remarkable cell and tissues specificity. Recently, alterations in a set of miRNAs in the brains of patients with certain neurodegenerative diseases, including prion diseases, have been reported. In this study, using deep sequencing technology, miRNA expression profiles in the brains of mice infected with scrapie agents 139A, ME7 and S15 at a terminal stage were comparatively analysed. In total, 57, 94 and 135 differentially expressed miRNAs were identified in the pooled brain samples of 139A-, ME7- and S15-infected mice, respectively, compared with the brains of age-matched normal controls. Among them, 22 were commonly increased and 14 were commonly decreased in the brains of all three infected models. In addition, a reduction in the expression of two novel miRNAs was also commonly observed. Quantitative PCR with reverse transcription analysis of six randomly selected commonly increased and decreased miRNAs in the brains of the three infected mouse models, as well as the two novel miRNAs, verified that the expression patterns were comparable to the deep sequencing data. KEGG analysis of the differentially expressed miRNAs revealed the involvement of similar pathways in all three types of infected animals. Comprehensive analysis of these miRNA profiles not only provides useful clues for understanding prion biology but also is beneficial in the search for possible diagnostic marker(s) for prion diseases.Emerging Microbes & Infections(2016)5,e115; doi:10.1038/emi.2016.120; published online 9 November 2016
登录
查看更多内容
影响因子：
3.7
作者：
Song C;Fang J;Wang C;Guo L;Nicholas KK;Ma Z
通讯作者：
Ma Z
影响因子：
56.9
作者：
Kim, Jongpil;Inoue, Keiichi;Abeliovich, Asa
通讯作者：
Abeliovich, Asa
影响因子：
5.4
作者：
Shi, Qi;Xiao, Kang;Dong, Xiao-Ping
通讯作者：
Dong, Xiao-Ping
影响因子：
14.9
作者：
Bellingham SA;Coleman BM;Hill AF
通讯作者：
Hill AF
影响因子：
12.4
作者：
Cortez, Maria Angelica;Valdecanas, David;Welsh, James W.
通讯作者：
Welsh, James W.