Significance of CD133 as a cancer stem cell markers focusing on the tumorigenicity of pancreatic cancer cell lines.
Significance of CD133 as a cancer stem cell markers focusing on the tumorigenicity of pancreatic cancer cell lines.
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DOI：
10.4174/jkss.2011.81.4.263
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发表时间：
2011-10
影响因子：
--
通讯作者：
Moon HJ
中科院分区：
文献类型：
--
作者：
Lee HJ;You DD;Choi DW;Choi YS;Kim SJ;Won YS;Moon HJ
癌症干细胞假说指出，癌症生长和繁殖的能力取决于一小部分细胞。通过比较分析确定癌症干细胞标志物CD 133、CD 44和CD 24的意义，重点关注致瘤性。通过流式细胞术分析四种胰腺癌细胞系Capan-1、Mia-PACA-2、Panc-1和SNU-410的CD 133、CD 44和CD 24的表达。在非肥胖型糖尿病重度联合缺陷小鼠中，使用肿瘤体积和形成的肿瘤数量/注射次数比较致瘤性。使用流式细胞术（FACS）分析来确认异种移植外植体来源于人胰腺癌细胞。CD 133仅在Capan-1中阳性，CD 44全部阳性，CD 24在Panc-1中部分阳性。在注射2 × 106个细胞后，所有给予Capan-1或Mia-Paca-2的小鼠均发生肿瘤，5只给予Panc-1的小鼠中有3只发生肿瘤，但给予SNU-410的小鼠均未发生任何肿瘤。Capan-1肿瘤的体积是Mia-Paca-2肿瘤的7倍。当注射2 × 105或2 × 104 Capan-1或Mia-Paca-2时，所有Capan-1处理的小鼠中均发生肿瘤，但Mia-Paca-2处理的小鼠中未发生肿瘤。此外，表达CD 133 + CD 44+的Capan-1的异种移植外植体和Capan-1注射的小鼠发生肺转移。流式细胞仪分析表明，异种移植外植体来源于人胰腺癌细胞系。CD 133阳性细胞的致瘤性和转移潜能高于CD 44和CD 24阳性细胞，提示CD 133可能是胰腺癌干细胞的一个有意义的细胞表面标志物。
The cancer stem cell hypothesis states that the capacity of a cancer to grow and propagate is dependent on a small subset of cells. To determine the significances of the cancer stem cell markers CD133, CD44, and CD24 using a comparative analysis with a focus on tumorigenicity. Four pancreatic cancer cell lines, Capan-1, Mia-PACA-2, Panc-1, and SNU-410 were analyzed for the expressions of CD133, CD44, and CD24 by flow cytometry. The tumorigenicity was compared using tumor volumes and numbers of tumors formed/numbers of injection in nonobese diabetic severe combined deficiency mice. Fluorescence-activated cell sorting (FACS) analysis was used to confirm that xenograft explants originated from human pancreatic cancer cells. CD133 was positive in only Capan-1, CD44 positive in all, CD24 partially positive in Panc-1. After injecting 2 × 106 cells, all mice administered Capan-1 or Mia-Paca-2 developed tumors, 3 of 5 administered Panc-1 developed tumors, but no mouse administered SNU-410 developed any tumors. The volumes of Capan-1 tumors were seven times larger than those of Mia-Paca-2 tumors. When 2 × 105 or 2 × 104 of Capan-1 or Mia-Paca-2 was injected, tumors developed in all Capan-1 treated mice, but not in Mia-Paca-2 treated mice. Furthermore, xenograft explants of Capan-1 expressed CD133+CD44+ and Capan-1 injected mice developed lung metastasis. FACS analysis showed that xenograft explants originated from human pancreatic cancer cell lines. CD133 positive cells have higher tumorigenic and metastatic potential than CD44 and CD24 positive cells, which suggests that CD133 might be a meaningful cell surface marker of pancreatic cancer stem cells.
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影响因子：
64.8
作者：
O'Brien, Catherine A.;Pollett, Aaron;Dick, John E.
通讯作者：
Dick, John E.
影响因子：
11.2
作者：
Galli, R;Binda, E;Vescovi, A
通讯作者：
Vescovi, A
DOI：
10.1186/bcr1855
发表时间：
2008
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Wright MH;Calcagno AM;Salcido CD;Carlson MD;Ambudkar SV;Varticovski L
通讯作者：
Varticovski L
影响因子：
11.2
作者：
Li, Chenwei;Heidt, David G.;Simeone, Diane M.
通讯作者：
Simeone, Diane M.
影响因子：
8
作者：
Patrawala, L;Calhoun, T;Tang, DG
通讯作者：
Tang, DG