Hepatocyte Ploidy Is a Diversity Factor for Liver Homeostasis.
Hepatocyte Ploidy Is a Diversity Factor for Liver Homeostasis.
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DOI：
10.3389/fphys.2017.00862
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发表时间：
2017
影响因子：
4
通讯作者：
Bartolomé-Rodríguez MM
中科院分区：
文献类型：
--
作者：
Kreutz C;MacNelly S;Follo M;Wäldin A;Binninger-Lacour P;Timmer J;Bartolomé-Rodríguez MM
多倍体，即含有一对以上染色体的细胞的存在，是哺乳动物肝细胞的一个众所周知的特征。多倍体肝细胞既有单个多倍体细胞核的细胞，也有二倍体甚至多倍体细胞核的多核细胞。在这项研究中，我们通过计算DNA含量和每个细胞的核数量来评估小鼠肝脏的多倍体程度。我们证明，与多倍体肝细胞相比，二倍体小鼠肝细胞具有不同的代谢特征。除了强烈的差异基因表达，包括代谢和信号化合物，我们发现核多倍体细胞的胰岛素结合强烈减少。我们的观察与细胞核倍性有关，但与细胞内的总倍性无关。因此，我们建议核倍性作为一种新的肝细胞多样性因子，并假设多倍体肝细胞可能具有与单核肝细胞不同的生物学功能。这种多样性独立于众所周知的与细胞沿肝门中心轴的位置有关的异质性。
Polyploidy, the existence of cells containing more than one pair of chromosomes, is a well-known feature of mammalian hepatocytes. Polyploid hepatocytes are found either as cells with a single polyploid nucleus or as multinucleated cells with diploid or even polyploid nuclei. In this study, we evaluate the degree of polyploidy in the murine liver by accounting both DNA content and number of nuclei per cell. We demonstrate that mouse hepatocytes with diploid nuclei have distinct metabolic characteristics compared to cells with polyploid nuclei. In addition to strong differential gene expression, comprising metabolic as well as signaling compounds, we found a strongly decreased insulin binding of nuclear polyploid cells. Our observations were associated with nuclear ploidy but not with total ploidy within a cell. We therefore suggest ploidy of the nuclei as an new diversity factor of hepatocytes and hypothesize that hepatocytes with polyploid nuclei may have distinct biological functions than mono-nuclear ones. This diversity is independent from the well-known heterogeneity related to the cells' position along the porto-central liver-axis.
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影响因子：
56.9
作者：
Bailey, JA;Gu, ZP;Eichler, EE
通讯作者：
Eichler, EE
影响因子：
56.9
作者：
MASTERSON, J
通讯作者：
MASTERSON, J
影响因子：
3.7
作者：
De Santis Puzzonia M;Cozzolino AM;Grassi G;Bisceglia F;Strippoli R;Guarguaglini G;Citarella F;Sacchetti B;Tripodi M;Marchetti A;Amicone L
通讯作者：
Amicone L
影响因子：
5
作者：
Engel, Felix B.;Schebesta, Michael;Keating, Mark T.
通讯作者：
Keating, Mark T.
影响因子：
3.7
作者：
CARRIERE, R
通讯作者：
CARRIERE, R