Histochemical structure and immunolocalisation of the hyaluronan system in the dromedary oviduct.
Histochemical structure and immunolocalisation of the hyaluronan system in the dromedary oviduct.
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单峰骆驼输卵管透明质酸系统的组织化学结构和免疫定位。
DOI：
10.1071/rd14187
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Mohey-Elsaeed O
中科院分区：
文献类型：
--
作者：
Mohey-Elsaeed O
我们研究了单峰骆驼 (Camelus dromedarius) 输卵管的一些组织化学特性的局部调节，重点关注壶腹和峡部中透明质酸 (HA) 合酶（HAS2 和 HAS3）、透明质酸酶（HYAL2 和 HYAL1）以及 HA 受体 CD44 的免疫定位。通过阿新蓝染色，沿纤毛和非纤毛上皮细胞 (LE) 的管腔表面检测到丰富的酸性粘多糖（糖胺聚糖）。与峡部相比，壶腹部初级上皮皱襞的 HAS2 染色较高，尤其是分泌细胞、腔内上皮表面和核上细胞区域。峡部 LE 中的 HAS3 染色比壶腹部更强。 HYAL2 在壶腹和峡部的 LE 中检测到，并且在分泌细胞的腔内投射中更为强烈。 HYAL1 在 LE 中检测到微弱，壶腹部和峡部之间没有差异。壶腹部和峡部的 LE 中存在强 CD44 免疫染色。 CD44 染色在分泌细胞中高于纤毛上皮细胞，并且在核上区高于细胞质基底区。总之，我们提供证据表明 HA 合成和周转发生在骆驼输卵管中。 HAS2 和 HAS3 表达的差异表明输卵管液中分泌的 HA 分子大小存在区域差异，这可能会影响骆驼输卵管与配子的相互作用。
We investigated the local modulation of some histochemical properties of oviducts of the dromedary (Camelus dromedarius), focusing on the immnolocalisation of hyaluronic acid (HA) synthases (HAS2 and HAS3), hyaluronidases (HYAL2 and HYAL1) and the HA receptor CD44 in the ampulla and isthmus. Abundant acidic mucopolysaccharides (glycosaminoglycans) were detected by Alcian blue staining along the luminal surface of both ciliated and non-ciliated epithelial cells (LE). Staining for HAS2 was higher in the primary epithelial folds of the ampulla compared with the isthmus, especially in secretory cells, adluminal epithelial surface and supranuclear cell domain. HAS3 staining was stronger in the LE of the isthmus than ampulla. HYAL2 was detected in the LE in the ampulla and isthmus and was more intense in the adluminal projections of secretory cells. HYAL1 was weakly detected in the LE with no difference between the ampulla and isthmus. Strong CD44 immunostaining was present in the LE of the ampulla and isthmus. CD44 staining was higher in secretory cells than in ciliated epithelial cells and was higher in the supranuclear region than the basal region of the cytoplasm. In conclusion, we provide evidence that HA synthesis and turnover occur in the camel oviduct. Differences in HAS2 and HAS3 expression suggest regional differences in the molecular size of HA secreted in oviductal fluid that may influence oviduct–gamete interaction in the camel.
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