Reciprocal transmission of herpes simplex virus type 1 (HSV‐1) between corneal epithelium and trigeminal neurites in an embryonic chick organ culture
Reciprocal transmission of herpes simplex virus type 1 (HSV‐1) between corneal epithelium and trigeminal neurites in an embryonic chick organ culture
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胚胎鸡器官培养物中角膜上皮和三叉神经突之间 1 型单纯疱疹病毒 (HSV-1) 的相互传播
DOI：
10.1096/fj.01-0803fje
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
J. Kühn
中科院分区：
文献类型：
--
作者：
W. Hafezi;B. Eing;E. Lorentzen;S. Thanos;J. Kühn
外周神经系统的上皮细胞和感觉神经元之间的相互传递是1型单纯疱疹病毒（HSV-1）和相关α疱疹病毒生命周期中的关键步骤。为了寻找一种易于执行且普遍适用的实验方法，从而能够直接分析原代上皮细胞和感觉神经突之间的病毒转移，我们研究了HSV-1在包含鸡胚角膜上皮和三叉神经感觉神经元的双室器官模型中的传播。发现鸡胚角膜和三叉神经组织允许HSV-1的生产性感染。我们的数据表明，HSV-1有效地进入重新支配角膜的神经突，并通过逆行轴突运输到达神经节外植体，感染后约12小时可检测到第一个抗原阳性细胞。直接感染三叉神经组织后，病毒通过顺行轴突运输运输至角膜上皮，在感染后约48 h引起可见的细胞病变效应。这些结果表明，在这项研究中提出的器官模型具有特殊的承诺，为直接观察和分子分析的单纯疱疹病毒之间的初级上皮细胞和感觉神经元的传播，它可能是一个有吸引力的替代目前的实验方法的基础上，实验室动物或人类胎儿组织。
Reciprocal transmission between epithelia and sensory neurons of the peripheral nervous system is a crucial step in the life cycle of herpes simplex virus type 1 (HSV‐1) and related alphaherpesviruses. In searching for an easy‐to‐perform and generally applicable experimental approach that enables the direct analysis of virus transfer between primary epithelial cells and sensory neurites, we investigated the spread of HSV‐1 in a dual‐chamber organ model comprising chick embryonic corneal epithelia and trigeminal sensory neurons. Embryonic chick corneal and trigeminal tissues were found to be permissive for productive infection with HSV‐1. Our data show that HSV‐1 efficiently enters neurites re‐innervating the cornea and reaches the ganglion explant by retrograde axonal transport, with the first antigen‐positive cells being detectable ~12 h postinfection. After direct infection of trigeminal tissues, the virus is transported by anterograde axonal transport to the corneal epithelium, causing a visible cytopathic effect ~48 h postinfection. These results suggest that the organ model presented in this study holds particular promise for the direct observation and molecular analysis of herpes simplex virus spread between primary epithelia and sensory neurons and that it may be an attractive alternative to current experimental approaches based on laboratory animals or human fetal tissues.
影响因子：
56.9
作者：
Geraghty, RJ;Krummenacher, C;Spear, PG
通讯作者：
Spear, PG