Enzyme-linked immunosorvent assay using vertical microreactor stack with microbeads
Enzyme-linked immunosorvent assay using vertical microreactor stack with microbeads
复制标题
使用带有微珠的垂直微反应器堆栈进行酶联免疫溶剂测定
DOI：
10.1007/s00542-008-0587-2
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Y. Utsumi
中科院分区：
文献类型：
--
作者：
Y. Ukita;T. Asano;K. Fujiwara;K. Matsui;M. Takeo;S. Negoro;Y. Utsumi
讨论了立体微反应堆在免疫分析中的优势。垂直微反应器堆使用具有多功能流体过滤器的垂直流体流动操作。流体过滤器的多功能性对于微混合和被动阀操作是非常有效的。利用计算流体力学（CFD）对微观混合的机理进行了探讨，认为微观混合的机理是基于柯恩达效应。为了评估流体过滤器的微观混合性能，我们展示了具有独特的重复混合操作的酶反应。结果表明，该过滤器具有很好的混合性能。竞争酶联免疫吸附试验（ELISA）检测限与日本环境省推荐的检测限相当。
The advantages of vertical microreactor stack with three-dimensional (3D) structure for immunoassay are discussed. The vertical microreactor stack uses vertical fluid flow operation with multifunctional fluid filters. The multi function of fluid filter is very effective for micromixing and passive valve operation. The mechanism of micromixing is discussed by using computational fluid dynamics (CFD), and we know that the mixing mechanism based on Coanda effect. To evaluate the micromixing performance of fluid filter, we demonstrated enzyme reaction with unique repeat mixing operation. As the results, we proved that the fluid filter has very effective mixing performance. The detection limit, which demonstrated by competition enzyme-linked immunosorvent assay (ELISA), is comparable with recommended detection limit, which suggested by Japanese ministry for the environment.
影响因子：
7.4
作者：
Anderson, JR;Chiu, DT;Whitesides, GM
通讯作者：
Whitesides, GM