A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants.
A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants.
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DOI：
10.1083/jcb.144.3.389
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发表时间：
1999-02-08
期刊：
影响因子：
--
通讯作者：
Simos G
中科院分区：
文献类型：
--
作者：
Hurt E;Hannus S;Schmelzl B;Lau D;Tollervey D;Simos G
To identify components involved in the nuclear export of ribosomes in yeast, we developed an in vivo assay exploiting a green fluorescent protein (GFP)-tagged version of ribosomal protein L25. After its import into the nucleolus, L25-GFP assembles with 60S ribosomal subunits that are subsequently exported into the cytoplasm. In wild-type cells, GFP-labeled ribosomes are only detected by fluorescence in the cytoplasm. However, thermosensitive rna1-1 (Ran-GAP), prp20-1 (Ran-GEF), and nucleoporin nup49 and nsp1 mutants are impaired in ribosomal export as revealed by nuclear accumulation of L25-GFP. Furthermore, overexpression of dominant-negative RanGTP (Gsp1-G21V) and the tRNA exportin Los1p inhibits ribosomal export. The pattern of subnuclear accumulation of L25-GFP observed in different mutants is not identical, suggesting that transport can be blocked at different steps. Thus, nuclear export of ribosomes requires the nuclear/cytoplasmic Ran-cycle and distinct nucleoporins. This assay can be used to identify soluble transport factors required for nuclear exit of ribosomes.
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影响因子：
64.5
作者：
Fornerod, M;Ohno, M;Mattaj, IW
通讯作者：
Mattaj, IW
影响因子：
7.8
作者：
Fahrenkrog, B;Hurt, EC;Panté, N
通讯作者：
Panté, N
影响因子：
64.5
作者：
BOGERD, HP;FRIDELL, RA;CULLEN, BR
通讯作者：
CULLEN, BR
影响因子：
4.8
作者：
IMAMOTO, N;TACHIBANA, T;YONEDA, Y
通讯作者：
YONEDA, Y
DOI：
10.1111/j.1432-1033.1996.0032t.x
发表时间：
1996-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Hassel, I;Cezanne, V;Fasold, H
通讯作者：
Fasold, H