Studies of lymphocyte reconstitution in a humanized mouse model reveal a requirement of T cells for human B cell maturation.
Studies of lymphocyte reconstitution in a humanized mouse model reveal a requirement of T cells for human B cell maturation.
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DOI：
10.4049/jimmunol.1202810
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发表时间：
2013-03-01
期刊：
影响因子：
--
通讯作者：
Pelanda R
中科院分区：
文献类型：
--
作者：
Lang J;Kelly M;Freed BM;McCarter MD;Kedl RM;Torres RM;Pelanda R
The hematopoietic humanized mouse (hu-mouse) model is a powerful resource to study and manipulate the human immune system. However, a major and recurrent issue with this model has been the poor maturation of B cells that fail to progress beyond the transitional B cell stage. Interestingly, a similar problem has been reported in transplant patients that receive cord blood stem cells. In this study, we characterize the development of human B and T cells in the lymph nodes (LNs) and spleen of BALB/c-Rag2nullIl2rγnull hu-mice. We find a dominant population of immature B cells in the blood and spleen early, followed by a population of human T cells, coincident with the detection of LNs. Notably, in older mice we observe a major population of mature B cells in LNs and in the spleens of mice with higher T cell frequencies. Moreover, we demonstrate that the T cells are necessary for B cell maturation, as introduction of autologous human T cells expedites the appearance of mature B cells, while in vivo depletion of T cells retards B cell maturation. The presence of the mature B cell population correlates with enhanced IgG and Ag-specific responses to both T-dependent and T-independent challenges, indicating their functionality. These findings enhance our understanding of human B cell development, provide increased details of the reconstitution dynamics of hu-mice, and validates the use of this animal model to study mechanisms and treatments for the similar delay of functional B cells associated with cord blood transplantations.
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影响因子：
6.4
作者：
Biswas, Subhabrata;Chang, Hong;Marasco, Wayne A.
通讯作者：
Marasco, Wayne A.
人T细胞的稳态膨胀和表型转化取决于与APC的外周相互作用。
DOI：
10.4049/jimmunol.0901711
发表时间：
2010-06-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Onoe T;Kalscheuer H;Chittenden M;Zhao G;Yang YG;Sykes M
通讯作者：
Sykes M
影响因子：
30.5
作者：
Manz, Markus G;Di Santo, James P
通讯作者：
Di Santo, James P
DOI：
10.1080/1024533031000072045
发表时间：
2003-02-01
期刊：
Hematology (Amsterdam, Netherlands)
影响因子：
--
作者：
Imamura, Masahiro;Tsutsumi, Yutaka;Tanaka, Junji
通讯作者：
Tanaka, Junji
DOI：
10.1016/j.bbmt.2011.11.014
发表时间：
2012-01
期刊：
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation
影响因子：
--
作者：
Bunin N;Small T;Szabolcs P;Baker KS;Pulsipher MA;Torgerson T
通讯作者：
Torgerson T