X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation.

X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation.
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低剂量照射后人类染色体的X射线重叠关联成像。

DOI:
10.1007/s10577-021-09660-7
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发表时间:
2021-03
期刊:
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
影响因子:
--
通讯作者:
Robinson IK
Robinson IK
中科院分区:
其他
文献类型:
--
作者:
Bhartiya A;Batey D;Cipiccia S;Shi X;Rau C;Botchway S;Yusuf M;Robinson IK

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对染色体在细胞遗传学中的结构和功能作用的研究已经跨越了10多年。在这项工作中,我们利用最新的同步辐射源的相干X射线提取的所有46条染色体的中期人类B和T细胞使用硬X射线重叠图的个人质量。我们已经制作了重金属染色和未染色的涂片的“X射线核型”,以确定由于辐射损伤引起的低水平X射线照射剂量下遗传物质的获得或损失。实验在I-13光束线,钻石光源,迪科,英国,使用相敏X射线重叠关联法进行。
Studies of the structural and functional role of chromosomes in cytogenetics have spanned more than 10 decades. In this work, we take advantage of the coherent X-rays available at the latest synchrotron sources to extract the individual masses of all 46 chromosomes of metaphase human B and T cells using hard X-ray ptychography. We have produced ‘X-ray karyotypes’ of both heavy metal–stained and unstained spreads to determine the gain or loss of genetic material upon low-level X-ray irradiation doses due to radiation damage. The experiments were performed at the I-13 beamline, Diamond Light Source, Didcot, UK, using the phase-sensitive X-ray ptychography method.
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