Young plasma attenuates cognitive impairment and the cortical hemorrhage area in cerebral amyloid angiopathy model mice.
Young plasma attenuates cognitive impairment and the cortical hemorrhage area in cerebral amyloid angiopathy model mice.
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年轻血浆减轻脑淀粉样血管病模型小鼠的认知障碍和皮质出血面积
DOI：
10.21037/atm-20-8008
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发表时间：
2021-01
影响因子：
--
通讯作者：
Lian H
中科院分区：
文献类型：
--
作者：
Li N;Zhang X;Gu Z;Su C;Lian H
脑淀粉样血管病（Cerebral amyloid angioopathy， CAA）的特征是β-淀粉样蛋白（β）在小脑膜血管和穿透小动脉中沉积。脑出血是CAA最具破坏性的并发症之一。年轻血浆已被证明可以改善阿尔茨海默病（AD）模型小鼠的认知、学习和记忆功能，是一种新的潜在治疗方法。然而，对于CAA小鼠APP/PS1（表达APP695swe和PS1- de9突变），年轻血浆是否能减少脑出血，改善神经功能预后尚不清楚。采用y型迷宫、新目标识别（NOR）、强迫游泳、开阔场地、蔗糖消耗和角落测试评估CAA模型小鼠的学习记忆、认知能力和情绪变化。采用免疫荧光法分析幼体血浆对神经发生的影响。采用酶联免疫吸附法（ELISA）检测小鼠大脑皮层和海马组织中Aβ的含量。最后用快速蓝染色法分析小鼠皮质出血面积。我们证明了年轻血浆改善了CAA模型小鼠的认知、学习和记忆障碍以及焦虑，阻止了APP/PS1小鼠的神经元凋亡，并促进了神经发生。然而，年轻血浆并没有降低APP/PS1小鼠皮质和海马中的Aβ水平。我们还发现，年轻血浆减少了APP/PS1小鼠脑出血的面积。我们的研究结果表明，年轻血浆可以改善CAA模型小鼠的学习记忆、认知障碍和焦虑，并可以减少皮质出血面积。
Cerebral amyloid angiopathy (CAA) is characterized by the deposition of β-amyloid (Aβ) in leptomeningeal vessels and penetrating arterioles. Intracerebral hemorrhage (ICH) is one of the most destructive complications in CAA. Young plasma has been shown to improve cognitive, learning, and memory functions in Alzheimer’s disease (AD) model mice and is a new potential therapy. However, it is not clear whether young plasma can reduce cerebral hemorrhage and improve the prognosis of neurological function in APP/PS1 (which express APP695swe and PS1-dE9 mutations) mice with CAA disease. The Y-maze, new object recognition (NOR), forced swimming, open field, sucrose consumption, and corner tests were used to evaluate the learning and memory, cognitive ability, and emotional changes in CAA model mice. The effect of young plasma on neurogenesis was analyzed by immunofluorescence. The level of Aβ in the cerebral cortex and hippocampus of mice was measured by enzyme-linked immunosorbent assay (ELISA). Finally, the area of cortical hemorrhage in mice was analyzed by fast blue-staining. We proved that young plasma improved cognition, learning and memory impairment, and anxiety in CAA model mice, prevented neuronal apoptosis, and enhanced neurogenesis in APP/PS1 mice. However, young plasma did not reduce the level of Aβ in the cortex and hippocampus of APP/PS1 mice. We also found that young plasma reduced the area of cerebral hemorrhage in APP/PS1 mice. Our results show that young plasma can improve learning and memory, cognitive impairment, and anxiety in CAA model mice and can reduce the area of cortical hemorrhage.
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影响因子：
64.8
作者：
Conboy, IM;Conboy, MJ;Rando, TA
通讯作者：
Rando, TA
DOI：
10.1073/pnas.0600948103
发表时间：
2006-03-28
影响因子：
11.1
作者：
Jacobsen, JS;Wu, CC;Bloom, FE
通讯作者：
Bloom, FE
影响因子：
4.2
作者：
Rockenstein, E;Mallory, M;Masliaha, E
通讯作者：
Masliaha, E
影响因子：
9.9
作者：
Knudsen, KA;Rosand, J;Greenberg, SM
通讯作者：
Greenberg, SM
影响因子：
17.1
作者：
Iliff JJ;Wang M;Liao Y;Plogg BA;Peng W;Gundersen GA;Benveniste H;Vates GE;Deane R;Goldman SA;Nagelhus EA;Nedergaard M
通讯作者：
Nedergaard M