Exploiting the ZIP4 homologue within the wheat Ph1 locus has identified two lines exhibiting homoeologous crossover in wheat-wild relative hybrids.
Exploiting the ZIP4 homologue within the wheat Ph1 locus has identified two lines exhibiting homoeologous crossover in wheat-wild relative hybrids.
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DOI：
10.1007/s11032-017-0700-2
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Moore G
中科院分区：
文献类型：
--
作者：
Rey MD;Martín AC;Higgins J;Swarbreck D;Uauy C;Shaw P;Moore G
尽管六倍体小麦具有相关的祖先基因组，但在减数分裂过程中表现为二倍体。小麦PH1基因座促进同源染色体的精确联会和交叉。虽然六倍体小麦与野生近缘杂种在中期I减数分裂过程中不会发生杂交，但在PH1缺失突变体与野生近缘杂种中，PH1缺失突变体与野生近缘杂种确实发生了杂交。在过去的40年里，已经开发了一个PH1缺失(PH1b)突变体来进行这一活动。我们在这里展示了化学诱导的突变株系，被选为在PH1基因座内的TaZIP4-B2突变，当与野生近缘杂交时，显示出高水平的同源交叉。Tazip4-B2突变系在多个世代中可能更稳定，因为导致染色体易位积累的多价体较少出现。因此，利用这样的Tazip4-B2突变体，而不是具有完整PH1基因座缺失的突变体，可能会改善野生相对染色体片段向小麦的导入。本文的在线版本(doi：10.1007/s11032-01700700-2)包含补充材料，授权用户可以使用。
Despite possessing related ancestral genomes, hexaploid wheat behaves as a diploid during meiosis. The wheat Ph1 locus promotes accurate synapsis and crossover of homologous chromosomes. Interspecific hybrids between wheat and wild relatives are exploited by breeders to introgress important traits from wild relatives into wheat, although in hybrids between hexaploid wheat and wild relatives, which possess only homoeologues, crossovers do not take place during meiosis at metaphase I. However, in hybrids between Ph1 deletion mutants and wild relatives, crossovers do take place. A single Ph1 deletion (ph1b) mutant has been exploited for the last 40 years for this activity. We show here that chemically induced mutant lines, selected for a mutation in TaZIP4-B2 within the Ph1 locus, exhibit high levels of homoeologous crossovers when crossed with wild relatives. Tazip4-B2 mutant lines may be more stable over multiple generations, as multivalents causing accumulation of chromosome translocations are less frequent. Exploitation of such Tazip4-B2 mutants, rather than mutants with whole Ph1 locus deletions, may therefore improve introgression of wild relative chromosome segments into wheat. The online version of this article (doi:10.1007/s11032-017-0700-2) contains supplementary material, which is available to authorized users.
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