Tissue distribution of inducible aldehyde dehydrogenase activity in the rat after treatment with phenobarbital or methylcholanthrene.
Tissue distribution of inducible aldehyde dehydrogenase activity in the rat after treatment with phenobarbital or methylcholanthrene.
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用苯巴比妥或甲基胆蒽治疗后大鼠体内诱导醛脱氢酶活性的组织分布。
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
M. Marselos
中科院分区：
文献类型：
--
作者：
V. Vasiliou;M. Marselos
本文用Wistar大鼠两个不同的亚系（RR和rr）研究了乙醛脱氢酶（ALDH）的组织分布。就肝ALDH活性的诱导而言，RR亚株对苯巴比妥（PB）有反应，而rr亚株被剥夺了这种生化特性。然而，这两个亚株，响应与甲基胆蒽（MC）的治疗，表现出一致的ALDH活性在肝脏中的增加。已知PB和MC诱导两种不同的肝细胞质同工酶。在RR亚株中检测PB（1g/l在饮用水中，持续12天）对肝外组织中ALDH的诱导作用。相反，对rr动物给予MC（50 mg/kg x 4，腹膜内）。用NAD和丙醛（P/NAD）或苯乙醛（Ph/NAD）测定PB诱导的肝和肠粘膜ALDH活性。当用NADP和苯甲醛（B/NADP）测量ALDH时，也注意到活性的增加。在RR动物中，MC诱导肝、肠粘膜、肾、肺、脾、脑、膀胱和心脏中的B/NADP活性。当以P/NAD或Ph/NAD活性来衡量ALDH时，MC对各种组织的影响不太明显。结果表明，PB和MC不仅诱导不同类型的ALDH活性，而且它们还揭示了ALDH诱导物的组织分布差异。
Two genetically distinct substrains of the Wistar rat (RR and rr) were used to study the tissue distribution of the inducibility of aldehyde dehydrogenase (ALDH). The RR substrain is responsive to phenobarbital (PB), as far as the induction of the hepatic ALDH activity is concerned, whereas the rr substrain is deprived of this biochemical property. Both substrains, however, respond to treatment with methylcholanthrene (MC), exhibiting a uniform increase of the ALDH activity in the liver. It is known that PB and MC induce two different isozymes of the hepatic cytosol. The effect of PB (1 g/l in drinking water, for 12 days) on the inducibility of ALDH in extrahepatic tissues was examined in the RR substrain. On the contrary, MC was given (50 mg/kg x 4, intraperitoneally) to rr animals. The activity of ALDH was found to be induced by PB in the liver and the intestinal mucosa, when measured with NAD and propionaldehyde (P/NAD) or phenylacetaldehyde (Ph/NAD). An increase of the activity was also noticed when ALDH was measured with NADP and benzaldehyde (B/NADP). In rr animals, MC induced the B/NADP activity in the liver, the intestinal mucosa, the kidneys, the lungs, the spleen, the brain, the urinary bladder and the heart. The effect of MC on various tissues was less distinct, when ALDH was measured as P/NAD or Ph/NAD activity. It is concluded, that PB and MC not only induce different types of ALDH activity, but they also reveal differences in the tissue distribution of the inducibility of ALDH.
正常大鼠肝脏中的过氧化物酶体相关乙醛脱氢酶。
DOI：
10.1016/s0091-3057(80)80018-9
发表时间：
1980
期刊：
Pharmacology, biochemistry, and behavior
影响因子：
--
作者：
Smith,HE;Lindahl,R
通讯作者：
Lindahl,R
来自大鼠肝细胞质的诱导型乙醛脱氢酶。
影响因子：
3.8
作者：
Simpson,VJ;Baker,R;Deitrich,RA
通讯作者：
Deitrich,RA
五种大鼠肝癌细胞系中乙醛脱氢酶活性的调节。
影响因子：
11.2
作者：
Lin,KH;Winters,AL;Lindahl,R
通讯作者：
Lindahl,R