Microbial carriage state of peripheral blood dendritic cells (DCs) in chronic periodontitis influences DC differentiation, atherogenic potential.
Microbial carriage state of peripheral blood dendritic cells (DCs) in chronic periodontitis influences DC differentiation, atherogenic potential.
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DOI：
10.4049/jimmunol.1201053
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发表时间：
2012-09-15
期刊：
影响因子：
--
通讯作者：
Cutler CW
中科院分区：
文献类型：
--
作者：
Carrion J;Scisci E;Miles B;Sabino GJ;Zeituni AE;Gu Y;Bear A;Genco CA;Brown DL;Cutler CW
慢性牙周炎（CP）是一种低度口腔感染性疾病，但其发病机制尚不清楚。在这里，血液树突状细胞（DC）在口腔粘膜病原体向动脉粥样硬化斑块的系统传播中的病理生理作用进行了研究。在患有或不患有急性冠状动脉综合征（ACS）的CP受试者和健康对照（CTL）中分析了CD 19 −BDCA-1+DC-SIGN+血髓样DC（mDC）的频率和微生物组。流式细胞仪分析显示血中mDC的显著增加，顺序如下：CTL<CP<ACS/CP。通过16 s rDNA测序分析血液mDC微生物组显示牙龈卟啉单胞菌和其他物种，包括（可培养的）洋葱伯克霍尔德菌。mDC携带牙龈卟啉单胞菌的比率与CP受试者的口腔携带率和牙龈卟啉单胞菌的血清学暴露相关。引起菌血症的干预（局部清创）增加了体内mDC携带率和频率。体外研究证实，牙龈卟啉单胞菌促进单核细胞向未成熟mDC分化28%;此外，mDC分泌高水平的MMP-9并以菌毛依赖性方式响应牙龈卟啉单胞菌上调C1 q、HSP 60、HSP-70、CCR 2和CXCL 16转录物。此外，当在mDC内时，需氧条件下厌氧菌牙龈卟啉单胞菌的存活增强。口腔粘膜和动脉粥样硬化斑块的免疫荧光分析表明与牙龈卟啉单胞菌共定位的mDC浸润。我们的研究结果表明，血液mDC在窝藏和传播病原体从口腔粘膜动脉粥样硬化斑块，这可能提供了mDC分化和动脉粥样硬化转化的关键信号的作用。
The low grade oral infection chronic periodontitis (CP) has been implicated in coronary artery disease risk, but the mechanisms are unclear. Here, a pathophysiological role for blood dendritic cells (DCs) in systemic dissemination of oral mucosal pathogens to atherosclerotic plaques was investigated in humans. The frequency and microbiome of CD19−BDCA-1+DC-SIGN+ blood myeloid DCs (mDCs) were analyzed in CP subjects with, or without existing acute coronary syndrome (ACS) and in healthy controls (CTL). FACS analysis revealed a significant increase in blood mDCs in the following order: CTL<CP<ACS/CP. Analysis of the blood mDC microbiome by 16s rDNA sequencing showed Porphyromonas gingivalis and other species, including (cultivable) Burkholderia cepacia. The mDC carriage rate with P. gingivalis correlated with oral carriage rate and with serologic exposure to P. gingivalis in CP subjects. Intervention (local debridement) to elicit a bacteremia increased the mDC carriage rate and frequency in vivo. In vitro studies established that P. gingivalis enhanced by 28% the differentiation of monocytes into immature mDCs; moreover, mDCs secreted high levels of MMP-9 and upregulated C1q, HSP60, HSP-70, CCR2 and CXCL16 transcripts in response to P. gingivalis in a fimbriae-dependent manner. Moreover, the survival of the anaerobe P. gingivalis under aerobic conditions was enhanced when within mDCs. Immunofluorescence analysis of oral mucosa and atherosclerotic plaques demonstrate infiltration with mDCs, colocalized with P. gingivalis. Our results suggest a role for blood mDCs in harboring and disseminating pathogens from oral mucosa to atherosclerosis plaques, which may provide key signals for mDC differentiation and atherogenic conversion.
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DOI：
10.1161/01.atv.0000121571.78696.dc
发表时间：
2004-04-01
影响因子：
8.7
作者：
Brown, DL;Desai, KK;Golub, LM
通讯作者：
Golub, LM
影响因子：
6.7
作者：
Distelmaier, Klaus;Adlbrecht, Christopher;Kubicek, Markus
通讯作者：
Kubicek, Markus
影响因子：
3.7
作者：
Alfakry, H.;Paju, S.;Pussinen, P. J.
通讯作者：
Pussinen, P. J.
DOI：
10.1084/jem.20092140
发表时间：
2010-06-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Jongbloed SL;Kassianos AJ;McDonald KJ;Clark GJ;Ju X;Angel CE;Chen CJ;Dunbar PR;Wadley RB;Jeet V;Vulink AJ;Hart DN;Radford KJ
通讯作者：
Radford KJ
影响因子：
4.3
作者：
Beck, JD;Offenbacher, S
通讯作者：
Offenbacher, S