Carbon monoxide modulates the response of human basophils to FcepsilonRI stimulation through the heme oxygenase pathway.
Carbon monoxide modulates the response of human basophils to FcepsilonRI stimulation through the heme oxygenase pathway.
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一氧化碳通过血红素加氧酶途径调节人类嗜碱性粒细胞对 FcepsilonRI 刺激的反应。
DOI：
10.1016/s0014-2999(03)01489-4
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发表时间：
2003
影响因子：
5
通讯作者：
E. Masini
中科院分区：
文献类型：
--
作者：
A. Vannacci;R. Baronti;G. Zagli;C. Marzocca;S. Pierpaoli;D. Bani;M. Passani;P. Mannaioni;E. Masini
我们报告了外源性和内源性一氧化碳（CO）对人嗜碱性粒细胞免疫激活的影响。血红素加氧酶底物类似物氯化血红素（1−100 μM）可显著增加部分纯化的人类嗜碱性粒细胞中胆红素的形成，从而表明这些细胞表达血红素加氧酶。用血红素加氧酶抑制剂Zn-原卟啉IX（100 μM）预孵育细胞30分钟可逆转该效应。氯化血红素（100 μM）还可降低免疫球蛋白G抗Fc ε（抗IgE）诱导的嗜碱性粒细胞活化，通过识别为簇分化蛋白63（CD 63）的膜颗粒相关蛋白的表达和组胺释放进行测量。这些作用可被锌原卟啉IX（100 μM）、一氧化碳清除剂氧合血红蛋白（HbO 2）（100 μM）和可溶性鸟苷酸环化酶抑制剂1H-[1，2，4]恶二唑并[4，3-a]喹喔啉-1-酮（ODQ）（100 μM）逆转。嗜碱性粒细胞暴露于外源性CO（10 μM，30分钟）也降低了它们的活化，而氮（N2）则无效。HbO 2和ODQ逆转了抑制作用，使膜蛋白CD 63表达和组胺释放均逆转至基础值。氯化血红素和外源性一氧化碳均能显著升高嗜碱性粒细胞cGMP水平，并减弱免疫激活引起的钙水平升高。这项研究表明，CO增加cGMP的形成，这反过来又诱导细胞内Ca 2+浓度的下降，从而导致人嗜碱性粒细胞活化的抑制。
We report the effects of exogenous and endogenous carbon monoxide (CO) on the immunological activation of human basophils. Hemin (1−100 μM), a heme oxygenase substrate analogue, significantly increased the formation of bilirubin from partially purified human basophils, thus indicating that these cells express heme oxygenase. This effect was reversed by preincubating the cells for 30 min with Zn-protoporphyrin IX (100 μM), a heme oxygenase inhibitor. Hemin (100 μM) also decreased immunoglobulin G anti-Fcε(anti-IgE)-induced activation of basophils, measured by the expression of a membrane granule-associated protein, identified as cluster differentiation protein 63 (CD63), and by histamine release. These effects were reversed by Zn-protoporphyrin IX (100 μM), by oxyhemoglobin (HbO2), a CO scavenger (100 μM), and by 1H-[1,2,4]oxadiazolo[4,3-a] quinoxalin-1-one (ODQ), an inhibitor of the soluble guanylyl cyclase (100 μM). Exposure of basophils to exogenous CO (10 μM for 30 min) also decreased their activation, while nitrogen (N2) was ineffective. HbO2and ODQ reversed the inhibition, reversing both membrane protein CD63 expression and histamine release to basal values. Both hemin and exogenous CO significantly raised cGMP levels in basophils and blunted the rise of calcium levels caused by immunological activation. This study suggests that CO increases cGMP formation, which in turn induces a fall in intracellular Ca2+concentration, thereby resulting in the inhibition of human basophil activation.
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DOI：
10.1016/s0034-5687(98)00072-3
发表时间：
1998
期刊：
Respiration physiology
影响因子：
--
作者：
Prabhakar,NR
通讯作者：
Prabhakar,NR
DOI：
10.1073/pnas.92.7.2568
发表时间：
1995-03-28
影响因子：
11.1
作者：
KHARITONOV, VG;SHARMA, VS;KOESLING, D
通讯作者：
KOESLING, D
影响因子：
20.1
作者：
Motterlini, R;Gonzales, A;Winslow, RM
通讯作者：
Winslow, RM
DOI：
10.1073/pnas.92.5.1475
发表时间：
1995-02-28
影响因子：
11.1
作者：
MORITA, T;PERRELLA, MA;KOUREMBANAS, S
通讯作者：
KOUREMBANAS, S
一氧化碳和一氧化氮同源性：大脑中血红素加氧酶的差异调节以及蛋白质和活性的检测。
影响因子：
--
作者：
Maines,M
通讯作者：
Maines,M