Amide proton transfer CEST of the cervical spinal cord in multiple sclerosis patients at 3T.
Amide proton transfer CEST of the cervical spinal cord in multiple sclerosis patients at 3T.
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在3T的多发性硬化症患者中，宫颈脊髓的酰胺质子转移。
DOI：
10.1002/mrm.26736
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发表时间：
2018-03
影响因子：
3.3
通讯作者：
Smith SA
中科院分区：
文献类型：
--
作者：
By S;Barry RL;Smith AK;Lyttle BD;Box BA;Bagnato FR;Pawate S;Smith SA
评价多发性硬化（MS）脊髓病理变化的能力有限，因为T1和T2-w MRI成像对体内生化变化不敏感。酰胺质子转移（APT）化学交换饱和转移（CEST）可以间接检测与蛋白质和肽相关的酰胺质子，潜在地提供更多的病理特异性。在这里，我们在3 T下在健康和MS队列的颈脊髓中实施APT CEST。在10名对照组和10名MS患者的队列中，使用一种新的呼吸校正方法获得颈脊髓的APT CEST。使用两种方法量化APT：1）APTw，基于常规磁化传递比（MTR）不对称性; 2）ΔAPT，MS患者相对于对照组的APT变化的空间表征。呼吸校正在白色物质中产生了高度可重复的z光谱（ICC=0.82）。MS患者的正常外观白色物质（NAWM）中的APTw信号与健康对照组有显著差异（P=0.04），而MS患者的ΔAPT图突出显示NAWM中的较大APT差异。脊髓中的呼吸校正对于准确量化APT CEST是必要的，APT CEST可以提供关于脊髓内疾病过程的独特生化信息。
The ability to evaluate pathological changes in the spinal cord in multiple sclerosis (MS) is limited, as T1- and T2-w MRI imaging is not sensitive to biochemical changes in vivo. Amide proton transfer (APT) chemical exchange saturation transfer (CEST) can indirectly detect amide protons associated with proteins and peptides, potentially providing more pathological specificity. Here, we implement APT CEST in the cervical spinal cord of healthy and MS cohorts at 3T. APT CEST of the cervical spinal cord was obtained in a cohort of 10 controls and 10 MS patients using a novel respiratory correction methodology. APT was quantified using two methods: 1) APTw, based off of the conventional magnetization transfer ratio (MTR) asymmetry and 2) ΔAPT, a spatial characterization of APT changes in MS patients relative to the controls. Respiratory correction yielded highly reproducible z-spectra in white matter (ICC=0.82). APTw signals in normal appearing white matter (NAWM) of MS patients were significantly different from healthy controls (P=0.04), while ΔAPT maps of MS patients highlighted large APT differences in NAWM. Respiration correction in the spinal cord is necessary to accurately quantify APT CEST, which can provide unique biochemical information regarding disease processes within the spinal cord.
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