Whole-genome expression profile in zebrafish embryos after chronic exposure to morphine: identification of new genes associated with neuronal function and mu opioid receptor expression.
Whole-genome expression profile in zebrafish embryos after chronic exposure to morphine: identification of new genes associated with neuronal function and mu opioid receptor expression.
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DOI：
10.1186/1471-2164-15-874
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发表时间：
2014-10-08
期刊：
影响因子：
4.4
通讯作者：
Rodríguez RE
中科院分区：
文献类型：
--
作者：
Herrero-Turrión MJ;Rodríguez-Martín I;López-Bellido R;Rodríguez RE
大量研究利用几种实验模型研究了吗啡暴露引起的基因表达的变化。在这项研究中，我们研究了斑马鱼中枢神经系统成熟和分化过程中长期暴露于吗啡过程中基因表达的变化。微阵列分析显示，与对照组相比，慢性吗啡暴露后，有254个基因的表达发生了至少1.3倍的变化。其中，几个新的基因(grb2、Copb2、otpb、magi1b、grik-L、bnip4和sox19b)在吗啡处理的实验动物模型中首次被检测到。我们还鉴定了慢性吗啡暴露后差异表达的一组基因(dao1、wls、bnip4和camk1γb)，它们的表达与Mu阿片受体基因的表达有关。COPB2、BNIP4、SOX19B、OTPB、DAO.1、GRIK-L和WLS的表达改变预示着神经元发育、中枢神经系统构型过程、分化和多巴胺能神经传递、5-羟色胺能信号通路和谷氨酸能神经传递。Camk1γb信号基因的失控提示轴突形成和树突形成的激活。我们的研究确定了与中枢神经系统发育相关的不同功能类别的基因和参与吗啡易感性机制的个人候选基因。这些结果为研究疼痛的治疗和成瘾的分子机制开辟了新的途径。我们还发现了一组斑马鱼特异的吗啡诱导基因，这些基因可能是人类成瘾和疼痛过程的假定靶点。本文的在线版本(DOI：10.1186/1471-2164-15-874)包含补充材料，可供授权用户使用。
A great number of studies have investigated changes induced by morphine exposure in gene expression using several experimental models. In this study, we examined gene expression changes during chronic exposure to morphine during maturation and differentiation of zebrafish CNS. Microarray analysis showed 254 genes whose expression was identified as different by at least 1.3 fold change following chronic morphine exposure as compared to controls. Of these, several novel genes (grb2, copb2, otpb, magi1b, grik-l, bnip4 and sox19b) have been detected for the first time in an experimental animal model treated with morphine. We have also identified a subset of genes (dao.1, wls, bnip4 and camk1γb) differentially expressed by chronic morphine exposure whose expression is related to mu opioid receptor gene expression. Altered expression of copb2, bnip4, sox19b, otpb, dao.1, grik-l and wls is indicative of modified neuronal development, CNS patterning processes, differentiation and dopaminergic neurotransmission, serotonergic signaling pathway, and glutamatergic neurotransmission. The deregulation of camk1γb signaling genes suggests an activation of axonogenesis and dendritogenesis. Our study identified different functional classes of genes and individual candidates involved in the mechanisms underlying susceptibility to morphine actions related to CNS development. These results open new lines to study the treatment of pain and the molecular mechanisms involved in addiction. We also found a set of zebrafish-specific morphine-induced genes, which may be putative targets in human models for addiction and pain processes. The online version of this article (doi:10.1186/1471-2164-15-874) contains supplementary material, which is available to authorized users.
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影响因子：
--
作者：
Del Giacco L;Sordino P;Pistocchi A;Andreakis N;Tarallo R;Di Benedetto B;Cotelli F
通讯作者：
Cotelli F
影响因子：
4.7
作者：
Charbogne P;Kieffer BL;Befort K
通讯作者：
Befort K
影响因子：
2
作者：
Fdez. de Velasco, Ezequiel Marron;Law, Ping Y.;Rodriguez, Raquel E.
通讯作者：
Rodriguez, Raquel E.
DOI：
10.1111/j.1530-0277.2012.01757.x
发表时间：
2012-09-01
影响因子：
3.2
作者：
Downing, Chris;Flink, Stephen;Kechris, Katerina J.
通讯作者：
Kechris, Katerina J.
新型脊髓背角星形胶质细胞 D-氨基酸氧化酶 - 参与吗啡镇痛耐受的过氧化氢途径的鉴定
影响因子：
8.8
作者：
Gong, Nian;Li, Xin-Yan;Wang, Yong-Xiang
通讯作者：
Wang, Yong-Xiang